Quantification of DNA! Hi, I explained the principle behind the extraction of pDNA in my last post. Extracted the DNA, what next? Let us quantify the DNA extracted. How to quantify? Very simple, all you need is Spectrophotometer. Just take 1 microliter of your DNA extract in an eppendorf, make it upto 500 microliter with nanopure water. (else 1000, or any desired volume) mix it well Now, put this into a quartz cuvette (0.5ml), measure the O.D value at 260 nm. Use blank as nano pure water. Note: The path length of the cuvette must be 1cm. Okay, we got the O.D value now, how to get the concentration of your DNA? It's very simple, do the following calculation. If your O.D. Value is 1, then, the concentration of your DNA sample is 50 microgram/ml so, now, if your concentration = obtained O.D.( let it be 0.5) * 50 microgram/ml * Dilution factor How to calculate the dilution factor? Simple! here your dilution factor is 500/1 i.e., 500 micro liter contains 1 micro liter of DNA, ...
