The Rushikesh Tahakik Blog

I recently published my first research paper in International Journal of research with IMPACT factor (5.6) not only my first in any journal, but also my first as a sole or lead author. I have never written an article for a scientific journal before, though I have experience of scribbling , here in this Blog,  Now, I know enough about how to to write a research article, How to handle research experiment,it's so exciting and fun to learn new things, I've done lots of mistake and learn so many new things also , till before i am not aware all this and  Today I am writing my first Research experience as a UG student here we go.... Yes there are long list of peoples who help me to sucess in writeing a research paper My professor, my juniors my sweet and cute classmate's, & my dear besties, Some FB and Whatsapp frds, and all my wellwishers thank  you very much all....  The path from idea to publication was long, winding and sometimes wandering off in the wrong d...

Introduction ?   okay let me introduce myself ... This is all about what i am Agricultural biotechnology student from MGM CABT,Aurangabad, Maharashtra I love learning and especially biology and I like to continouing basics in biology with biotechnology my ultimate AIM is utilise my knowledge which i receive from my masters degree so firest of all i have to complete my masters degree in any CENTERAL GOVT. institute and makes hone perfect my practical skills by work with an biotech industry and in future develop my own biotech industry and I hope i will make something that would add value in humans life I am not a genious, and not a average i am last bencher but not think i am poor in acedimics i m oneamoung topper in my class i always try to came out answers when prof. addressed a question my hand always raised with yesss ?? I cant remain  quite in my class i always asking dought about lecture and doing lecture with 100 concentration i am not narrate all this for blowing m...

The Mind Crunch Mimamsa : स्त्री, एक परिचय :                              पहन ली वो ढीली सी कमीज और पायजामा। अपने बेतरतीब बड़े बालो को छोटे छोटे काट नकार दिया मैंने लड़की होना। अचानक स...

वाडा चिरेबंदी ..... वाडा चिरेबंदी, तसा आजूनही आहे उभा आब-रुबाब उरला नाही घोडे-तांगे बारदाना गेला कळा गेली ,रया आली घोशा सुध्धा उघडा झाला वाडा चिरेबंदी तसा आजूनही आहे उभा शेतं ...

ऑलिम्पिकचं हे थीम साँग. केटी  पेरीनं गायलेलं. ते ऐकतानाच एक सळसळता उत्साह संचारतो काय नाही या गाण्यात? झुंजण्याची चिवट जिद्द आहे, कितीही छाटून टाकल्या फांद्या तरी मुळाप...

Quantification of DNA! Hi, I explained the principle behind the extraction of pDNA in my last post. Extracted the DNA, what next? Let us quantify the DNA extracted. How to quantify? Very simple, all you need is Spectrophotometer. Just take 1 microliter of your DNA extract in an eppendorf, make it upto 500 microliter with nanopure water. (else 1000, or any desired volume) mix it well Now, put this into a quartz cuvette (0.5ml), measure the O.D value at 260 nm. Use blank as nano pure water. Note: The path length of the cuvette must be 1cm. Okay, we got the O.D value now, how to get the concentration of your DNA? It's very simple, do the following calculation. If your O.D. Value is 1, then, the concentration of your DNA sample is 50 microgram/ml so, now, if your concentration = obtained O.D.( let it be 0.5) * 50 microgram/ml * Dilution factor How to calculate the dilution factor? Simple! here your dilution factor is 500/1 i.e., 500 micro liter contains 1 micro liter of DNA, ...