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Lowry Assay Principle and procedure

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Good evening all of you its nice to write hear my lab experience i.e. now become habbit to write this to me and now my  aim is to make perfect my practical skill because to hone perfect my overall grade points. Now i stop hear blowing my own trumpet and start for topic Thought there are several protein assays available,we do DNS last time but  the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.1 mg/ml to 1 mg/ml.(we use 1 mg per ml i.e. add 50 u in 50 ml water ) As  per procedure provide And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) (i goohled this ) Why Lowry? Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assa...

Caffeine-How much did u need ?

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Now a days, coffee comes in many varieties, such as Nescafe - Rubusta  and Bru- Gold , as well as different flavors and sizes. Colas are made with artificial flavors and added caffeine. The amount of caffeine in all of these different drinks can vary widely. Coffee used to be just "black". Coca-Cola was originally made with Kola nut extracts and contained cocaine ?? no wonder it was so popular! Energy drinks are a new trend in highly caffeinated beverages. They contain a wealth of sugar and other natural stimulants that help provide that sought-after energy boost. Caffeine is also found in many weight loss preparations and in some over-the-counter pain, diet and stimulant medications. Here are the most common sources of caffeine In India Coffee - Contains about 100mg per 250 ml. cup though most coffee . Black Tea - Contains 50mg per 250 ml Cup Green Tea  -contains 25mg. Caffeinated Sodas - Coke, Pepsi, and others contain 50-60 mg per 500 ml Super-Caffeinat...

First Few days in MGM-CABT

It was 20th june I believe! I just saw the details of the Maharashtra agriculture research institutions(MCAER) undergraduate programs only a week before the last date. I decided to apply for it and just applied in a hurry actually, yes, it was really a hurry! I was done with everything and sent the application. I was waiting for the release of selected list of candidates from the 25th july They updated the selection list everyday but my name was not on the list till 25th of March!that time i require admission is very essential otherwise my 1 year may spoil or if i took wrong decision may my whole carrier in danger to i remain very cool try to encourage myself because i fail pmt with narrow margin i dont want to took admission for BAMS or pharmacy . I used to go to the MCAER website everyday and check the list, but, my name was not there in the selected list of candidates. I lost all my hope and decided that I won't get selected. But, it happened! I received a mail from the Academ...

All I Needed to Know I Learned in ... ???

I know I am going off on a tangent here, away from my destination of a Creationist's view of biotechnology but I was thinking that there are many books and essays and teachings in the secular world that men use to guide their lives that either contain lessons similar to those in the Word or are directly or indirectly based on them. Three such books to me are: All I Really Need To Know I Read  in These Books  it is the "secret " and The Seven Habits of Highly Effective People by Stephen R. Covey Each has several key directives to focus on to make your life more relational, less anxious and guided by a purpose or destiny. Lets take a closer look at some of the directives from each book. All You Really Need to Know All I Really Need To Know secret is a book of  Rhonda Byrne , and book become so popular until it get in my hand, The title explain itself what is book actually about i.e. lists lessons normally learned in everyday life and some secret of great people...

Breathing My love

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Beginning......(Written My first science paper)

I recently published my first research paper in International Journal of research with IMPACT factor (5.6) not only my first in any journal, but also my first as a sole or lead author. I have never written an article for a scientific journal before, though I have experience of scribbling , here in this Blog,  Now, I know enough about how to to write a research article, How to handle research experiment,it's so exciting and fun to learn new things, I've done lots of mistake and learn so many new things also , till before i am not aware all this and  Today I am writing my first Research experience as a UG student here we go.... Yes there are long list of peoples who help me to sucess in writeing a research paper My professor, my juniors my sweet and cute classmate's, & my dear besties, Some FB and Whatsapp frds, and all my wellwishers thank  you very much all....  The path from idea to publication was long, winding and sometimes wandering off in the wrong d...

ABOUT ME

Introduction ?   okay let me introduce myself ... This is all about what i am Agricultural biotechnology student from MGM CABT,Aurangabad, Maharashtra I love learning and especially biology and I like to continouing basics in biology with biotechnology my ultimate AIM is utilise my knowledge which i receive from my masters degree so firest of all i have to complete my masters degree in any CENTERAL GOVT. institute and makes hone perfect my practical skills by work with an biotech industry and in future develop my own biotech industry and I hope i will make something that would add value in humans life I am not a genious, and not a average i am last bencher but not think i am poor in acedimics i m oneamoung topper in my class i always try to came out answers when prof. addressed a question my hand always raised with yesss ?? I cant remain  quite in my class i always asking dought about lecture and doing lecture with 100 concentration i am not narrate all this for blowing m...