Lowry Assay Principle and procedure
Good evening all of you its nice to write hear my lab experience i.e. now become habbit to write this to me and now my aim is to make perfect my practical skill because to hone perfect my overall grade points. Now i stop hear blowing my own trumpet and start for topic Thought there are several protein assays available,we do DNS last time but the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.1 mg/ml to 1 mg/ml.(we use 1 mg per ml i.e. add 50 u in 50 ml water ) As per procedure provide And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"??? :P) (i goohled this ) Why Lowry? Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assa...