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Showing posts from August, 2017

Lowry Assay Principle and procedure

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Good evening all of you its nice to write hear my lab experience i.e. now become habbit to write this to me and now my  aim is to make perfect my practical skill because to hone perfect my overall grade points. Now i stop hear blowing my own trumpet and start for topic Thought there are several protein assays available,we do DNS last time but  the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.1 mg/ml to 1 mg/ml.(we use 1 mg per ml i.e. add 50 u in 50 ml water ) As  per procedure provide And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) (i goohled this ) Why Lowry? Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assa...

Caffeine-How much did u need ?

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Now a days, coffee comes in many varieties, such as Nescafe - Rubusta  and Bru- Gold , as well as different flavors and sizes. Colas are made with artificial flavors and added caffeine. The amount of caffeine in all of these different drinks can vary widely. Coffee used to be just "black". Coca-Cola was originally made with Kola nut extracts and contained cocaine ?? no wonder it was so popular! Energy drinks are a new trend in highly caffeinated beverages. They contain a wealth of sugar and other natural stimulants that help provide that sought-after energy boost. Caffeine is also found in many weight loss preparations and in some over-the-counter pain, diet and stimulant medications. Here are the most common sources of caffeine In India Coffee - Contains about 100mg per 250 ml. cup though most coffee . Black Tea - Contains 50mg per 250 ml Cup Green Tea  -contains 25mg. Caffeinated Sodas - Coke, Pepsi, and others contain 50-60 mg per 500 ml Super-Caffeinat...