एक सुंदर आठवण....

जुन्या दिवाळीच्या सुट्यांची आठवण येतेय. पण डोळे मिटून आठवायचा प्रयत्न केला, आमचा वाडा वाड्यातील खनाची घर, बाजूला एक मोठ्ठ डेरेदार लिंबाचं झाड, वळणाचा रस्ता, नागमोडी नदी, मागे टेकडी आणि नदीत मावळताना सूर्याच्या केशरी छटा अस चित्र समोर आहे माझ्या. आणि त्या चित्रात नकळत कोणीतरी मलाही रंगवलंय. त्या शांतपणात माझ्या जाण्याने एक छोटासा तरंग उठतो, आणि बाकी सगळं पुन्हा शांत, सौम्य, गूढ.येतय डोळ्यासमोर ????
सगळं एका फिल्म सारखं येतंय डोळ्यासमोर
एक नउवार चापूनचोपून नेसलेली, केसांचा अंबाड़ा त्यात एखाद फूल, कानात कुड्या, हातात हिरवा चूड़ा, दोन पाटल्या, कमरेत चाव्यांचा जुड़गा, पायात जोडव्या,चेहऱ्यावर सुरकुत्या, डोळ्यांत आपल्या पाखरांची वाट आणि खुप माया. माझी आजी अगदी अशीच होती. खुप कमालीची आणि नेहमी हसतमुख. मी कधीही तिला थकलेली पहिलीच नाही. सतत कामात आणि तरीही अगदी शांत.
आम्ही लहानपणी जेव्हा गावाला जायचो , ऑक्टोबर महिन्यात तेव्हा एसटी तुन उतरल्या उतरल्या आम्ही आजीच्या घरापर्यंत ओरडत धावतच सुटायचो. मी तिला कायम घराच्या खिडकीतच पहायचो  जणू काही मागच्या मे महिन्यापासून  ती आमच्या वाटेकडे डोळे लावून तिथेच उभी आहे की काय? घरात शिरल्यावरची तिची ती उबदार मिठी आणि गालाचे घेतलेले पापे अजूनही अंगावर काटा आणतात. अबई !!!! म्हणायचो मी तिला घरी ती आम्हाला नाहुमाकू घालायची, आम्ही येणार म्हणून मुद्दाम आणलेली खारी ,अंगणात मुद्दाम बांधलेला झोपाळा,आमच्यासाठी केलेला फुलांचा गजरा, तिने आमच्यासाठी शिवलेली गोधडी जिची ऊब आजही तशीच आहे. सार काही मनाच्या एक स्पेशल कोरीव बॉक्स मधे जसच्या तसं आहे. तिने केलेली पुरणाची पोळी वर तुपाची धार, एक सवता बुंदीचा लाडु  सगळ्यांची चव अजूनही रेंगाळते आहे जिभेवर. देवापुढे दिवा लावून आमच्याकडून शुभंकरोति हट्टानी म्हणून  घ्यायची.
संध्याकाळी तुळशी वृंदावनसमोर दिवा लावणारी माझी आजी तीच ते मोहक रूप अजूनही डोळ्यासमोरुन जात नाही. रात्रीच्या गावातल्या भयंकर अंधारात आम्ही तिचा पदर पकडून तिच्या मागे मागे असायचो, आणि ती नुसतीच हसायची पण खरतर तिला हे आवडायच. 

बाईचे कसे वेगवेगळे वर्जन असतात किनई लहानपनीच हट्टी वागणं, तरुणपणी प्रेमात पडल्यावर अजून थोड़ मचुअर लग्नानांतर तर मचुरिटी च्या एज ला आणि आई झाल्यावर पूर्ण जवाबदार (मुलांच्या बाबतीत तरी 😁  ) आणी  आजी झाल्यावर जवाबदार च्या पलीकडे.  कुठून येत हे शहाणपण ? कोण शिकवत असेल हे ? आई नेव्हर अंडरस्टँड  ?? पुढच्या जन्मी स्त्रीचा जन्म घ्यावा लागेल !!
मुलगी असतांना हट्टी नाकावर राग असणारी पण नाजुक गर्लफ्रिएन्ड आणि बायको असताना हळुवार आणि समजूतदार तर आई होताना कठोऱ असणारी, आजी झाल्यावर कोमल कशी होते?                
माझ्या आजीच्या घराबाहेर गुलाबच मोठ झाड़ होत. (हो, झाड़च कारण ते झाड़ाएवढ मोठ होत ) आजी-आजोबा चारधाम ला गेले असताना आजीने हट्टान ते रोप घेतल होत. त्या गुलाबला एका वेळी किमान ७ ते ८ गुलाब यायचे. आई सांगते ते गावठी गुलाब होत, पण त्या गुलाबाचा सुगंध आजतागायत मला कुठेही मिळाला नाही. आपल्या सिटी लाइफ मधे बऱ्याच मॉल मधे त्या सुगंधाचा परफ्यूम मिळतो का म्हणून आजही शोधात फिरत असते पण अजुन ही नाही सापडला तो सुगंध. " खरच तो सुगंध गुलाबाचा होता की आजीच्या मायेचा होता ? तिच्या प्रेमाचा ? की तिच्या आठवणींचा ? " आजी गावावरुन आमच्या घरी येताना आठवणीने गुलाबाची फूले  आणायची. विशेष म्हणजे नंतर जेव्हा ती आजारी पडली तेव्हापासून झाड़ मलूल पडल. पुढे तीच आजारपण  वाढत गेल आणि एकाएकी झाडावर गुलाब येईनासे झाले. ती ज्यादिवशी गेली जानेवारी मधे तेव्हा झाड़ही गेल. हे अस कस? हे न सुटलेल कोड़ आहे.
माहीत नाही आपल्या नातवंडाना नऊवारी मधील आजी बघायला मिळेल की ती आता फ़क्त फोटोतच....  त्यांना त्या गोधडीची ऊब, तुळशी वृंदावनासमोरच्या त्या  दिव्याच मोहक रूप, शुभंकरोति ची गोड़ी, गावतला अंधार, अंधरातली नसलेली भुत,आजीच ते 12 खनाच घर आपल्यासाठी बांधलेला दोरीचा झूला,शेणानी सारवलेल अंगण आणि आजीच्या अंगणातला गुलाब... ह्या साऱ्याची मजा कधी कळणारच नाही.
आज आजी ही एक सुंदर आठवण आहे." त्या गुलाबाच्या सुगंधासारखी , हरवलेली तरीही जिवंत. "

Egg Facts

That's funny I remember when my parents buy fowl, and remembering my older sis cleaning the fowl we had found eggs of the fowl without the shell tho. And when we used the eggs for breakfast the yolk was orange always found that weird but it's naturally not weird after all
ActuallyEgg yolks should be a darker orange, as opposed to the bright yellow. The store brand egg light in color displays that the hen was malnourished, or fed unnatural chemicals/feed. Yolk, orange in color display more nutritional value of a positive and healthier diet feed

International Coffee day

Over the years, scientists have spent a lot of time looking at the beneficial effects of coffee, possibly because many of them seem to live off it. A recent reanalysis of existing studies suggests that people who drink 2 additional cups of coffee are 44% less likely to get excessive liver scarring. What is amazing is that this “protective effect” seem to increase with the amount of coffee you drink, and has been seen in multiple studies. However, it is still not clear exactly how coffee benefits the liver. More experiments still need to be done to find out if this benefit is due to caffeine or something else in coffee, and whether if the methods of preparation would make a difference.
Happy international coffee day!

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Further reading:
http://www.journal-of-hepatology.eu/article/S0168-8278(17)30147-2/fulltext
http://onlinelibrary.wiley.com/doi/10.1111/apt.13523/full
https://www.ncbi.nlm.nih.gov/pubmed/25291138
https://www.britishlivertrust.org.uk/liver-information/diet-and-liver-disease/coffee-and-the-liver/

Reason Why U read this blog ...

Good Morning ladies and gentleman
I have started this blog as a fresh start. A fresh start for me to be the more positive, stronger me that I have become over the past several years. I am 20, in a happy and single life and appearing B.sc agriculture biotechnology , and I Damm passionate towards my graduation
I am student, writer, blogger, I love singing, and singer ,books and author's, l I like readings , I can read for hours, I can talk for hours, I love whatever I do 😁

I am at my ultimate happiness high and I wish for you all to feel the same. The future is a fabulous thing which you can sculpt to fit you so don’t be scared of the future, embrace it.

This Blog is for you.

Whatever you want me to write about I will, to my best ability.
I am no guru(teacher) and I give terrible advice but I will always try. I am just a normal boy completed just teenage on the brink of creating a brand new life for herself. In a way, we’re all doing that everyday, creating a new life each day as we plod along. I am giving a conscious effort to make myself a new life, however.

I am keeping active (walking, doing Pilates, swimming gym on occasion etc.), trying to speak to new people, making this group  eating healthy (at least trying) and keeping positive in my mind. I am me and that’s okay. In fact, that’s more than okay, it’s fabulous! And it’s fabulous that you are you! Don’t let anyone tell you different, ever.

You may find this Blog all very cringey but that is not my intention. I want to embrace you to love yourself. I will equally love you all (unless you’re very mean to me, I may love you less then). I am not here to push you, hell I hardly push myself! I am just here as a helping hand to tell you that you’re going to be okay no matter what. I can be your friend, your mentor or just some of  you don’t care about. It’s up to you. You are the author of your own story.

HITLER AND BIOTECHNOLOGY

HITLER AND BIOTECHNOLOGY- Ultimate killing machine 

Nazi scientists secretly researched the possibility of dropping malaria-infected mosquitoes behind enemy lines during World War Two, a German academic has claimed

Hitler’s scientists planned to use malaria-infected mosquitoes as biological weapons by sending them behind enemy lines in the Second World War, according to research.

SS leader Heinrich Himmler ordered secret research into how insects infected with the disease could be kept alive long enough to be used against the allies, German academic Dr Klaus Reinhardt has claimed.

In January 1942, the SS chief created a special laboratory at Dachau concentration camp with the official aim of finding new remedies against diseases transmitted by lice and other insects, as well as the typhoid that plagued German troops.

But in the journal Endeavour, Reinhardt said records kept by Dachau Entomological Institute revealed its scientists also pursued research into biological warfare.

They tested the life spans of different mosquito breeds to try and find one that would remain alive long enough to be dropped into enemy territory.

At the end of the trials in 1944 a breed of anopheles mosquito, particularly effective in transmitting malaria to humans, was recommended by the director of the institute, according to Reinhardt, from Germany's Tuebingen University.

Image Source : INTERNET 

It is not known whether there was a connection between the work of the Entomological Institute and the chilling experiments carried out by Dr Claus Schilling who used prisoners as human guinea pigs deliberately infecting them with malaria.

Schilling was sentenced to death by hanging after the war.

The mosquito research had to remain secret because Germany, alongside the allied nations, had signed up to the 1925 Geneva protocol banning the use of biological and chemical weapons.

But Reinhardt dismissed the project as "a bizarre mix of Himmler's smattering of scientific knowledge, personal paranoia, an esoteric world view, and genuine concerns about his SS troops" which proved of no use to Hitler’s war effort

MEMORIES

Since childhood I am passionate about natural science. I grew up in a village surrounded by forests with many different animals and plants where my parents were the accomplices of my discoveries, transmitting their passion for observing and understanding nature. When it came time to decide on my academic orientation, I did not hesitate at any time to specialize me in the field of Biotechnology.

Biotechnology is, as a traditional definition, “the technique that allows the application of biology to make a profit”. Actually, all applied biology can be defined as Biotechnology, placing its origin in the technique of making beer in ancient Mesopotamia. The new vaccines, treatments or stem cell gene therapy, are Biotechnology. Transgenic crops or production of insulin for diabetics, are Biotechnology. Currently, the development of new biotechnology, thanks to genetic engineering, is so great that it has come to be called “the science of the future”.

Traditionally, Biotechnology is divided in five colors, each one corresponding to an area of ​​application:

• Red Biotechnology, applied to medicine. Stem cells or gene therapy are clear examples of Red Biotechnology.
• White Biotechnology, applied to industry. This is the case of the production of insulin for diabetics.
• Green Biotechnology, applied to agriculture, as in the case of GM crops or new pest control systems.
• Gray Biotechnology, applied to environmental protection, a good example is the bacteria used to degrade oil.
• Blue Biotechnology, an obsolete term for marine applications of Biotechnology.

Biotechnology is an exciting universe that mixes in a surprising way mysteries and certitudes. Studying Biotechnology Grade I became aware of the importance of science and began to look around me in a different light… And very quickly I became passionate for Biotechnology! It cleared me the way to life and general scientific knowledge (Physics of biological processes, General Chemistry, Organic Chemistry…) and then delving into some areas of biology (Genetics, Microbiology, Biochemistry …) and finally treating biotechnology issues (Agricultural Biotechnology, Applied Microbiology …).

During my career I had the opportunity to meet with renowned researchers and professionals who contributed to making me a professional excited by science.

Currently I have the good fortune to work in this field and I am very proud to have dedicated my career to several projects that will changed my life. It is a privilege to work on what you like and what you studied. Also, it is essential for me to continue increasing my creativity and improving my knowledge to follow the technological advances of science. Like many professionals of nowadays, leaving the routine and freshened up towards a new direction

Thank you

Tahakik rushi

What I learn ....

This is all about "what I learn!" I'm a B.sc. agri. (Biotechnology) student from Marathawada krushi universityand  MGM

college of ag.Biotechnology, gandheli, aurangabad, maharashtra , India. I love learning and I'm happy with learning. Still continuing with the basics of biotechnology; I'am not a genius; I read, I know something about my subject; So, you do can expect something useful from me! I love my teachers; I'm a keen observer, so you can expect some good stuffs for learning from me. If you are a starter in the course of biology/biotechnology/in general life science, who enjoys fun, who is eager to learn, you would love me!!!!

I'm a last bencher in class, but, don't think that i'm poor in my academics, I'm one among the toppers of my class. And, I'm the one who always try to come out with answers when the professor addresses some questions. I love teaching my own classmates, Chatter box...! I use to be the first to say "yes", when the teacher asks for taking a practical. The reason for blowing my own trumpet is to give you hope that you could expect some useful stuffs from me :) :) :D :P
i would like to share my personal experience , what I feel, what I love!
I'm sharing my lab experiences, mistakes, rectifications here, because it might help someone who search for any doubts in biotechnology like basics, simple silly and useful doubts. I have lots of useful stuffs for bio learning over here.

There may be mistakes/errors because I share only the things I understood (sometimes, I may go wrong in understanding certain complex things) But, I take much care to provide error free material (up to my knowledge)

Feel free to read, Feel free to contact me for very basic biotech doubts. And one more thing, my favorite papers are "Microbiology", "cell biology", "Molecular biology", "Genetic Engineering", "Immunology", "Plant biotechnology", "Animal Biotechnology". I love microbes, horticulture, agronomy, .

Loads of honey for you...!!! Enjoy tasting the sweetness of Learning Biotechnology!
Cheers!! :D :)

On writting - Monsters are real, and ghosts are real too. They live inside us, and sometimes, they win.

Writing is socially acceptable form of being naked in public – Paulo Coelho

When I read this quote, I was dumb founded! I always shied away from sharing my writings as I was not comfortable baring my thoughts or feelings..I used to think if someone reads this, what would they think? If I write something about love / pain would they think I have experienced it myself? I was not ready to answer anyone’s questions if they did! I wrote still but not about anything personal. I wrote for my diary/ on Facebook / WhatsApp group /hobby.

But everything was about some snippet seems like political my stuff most of the time. If I posted anything that would look personal political , my inbox would be flooded with oh, what happened, etc.
After I read this quote, I was like if such a great author can feel this way, it was ok for me to feel the same!

It is time now to write about what I really want to write without having any inhibitions..It may not be liked by all…but I would love the freedom of having all the thoughts outside my mind onto my group / fb / article medium.

‘Coz I have also learnt that when you put out all your thoughts on paper it reduces the intensity of your feelings..you become more calm & are at peace with yourself.

I would be very happy to unleash my love / passion for writing & also sharing it with few people who are interested in my work.

So how did it all began?

It all started with my another passion, Reading! Ya right, If I do anything, it has to be passionately! I don’t know any other way to do it 🙂

Reading has always been like a passion for me since childhood. I have always been a voracious reader. It may be newspapers, comics, short stories, novellas or full fledge novels. I forget myself when i am reading.. I become one with the characters & feel their emotions empathetically. It takes me to places where I can not physically go..transporting me to a world of imagination. Reading has also fuelled my already active imagination! The next logical step for me was to then pour out all the imagination in the form of writing..

I used to write poetries, short essays & stories..but I immediately tore them up after writing..(but aftet Apart from the reason above, I was also not sure whether I was a good writer or not..

Getting appreciated for whatever I have written till now has made me more comfortable with sharing my writings with public..

To cut a long story short, it is time to fuel my passion & drive it on the highway of imagination..If I get some friends along with me, cool!! If not, then Ekla Chalo Re!!!

University Practical exams

MY VIVA #1
Hi,
During the last practical exams I shared my experience. I shared certain questions with answers which the examiner asked me during the practicals.even i share what i learn  Even this time, I'm going to share a few questions and answers with you. Here we go,

This time I had  exam (Practicals), number  - microbial genetics and yesterday enzymology in biochemistry and - Molecular biology.
Let us start with the Biochemistry and molecular Biotechnology lab.

1.microbial genetics

What I know about streaking :

Purpose of streaking : 
Why to Streak?

1) To get isolated colonies. ( mostly bacteria )
2) To check the presence of microbes in a sample ( sometimes)

Types:

1) Quadrant streaking: The plate is divided into 4 quadrants and streaking is done.
2) Continuous streaking: Continuous streak made from top to bottom.
After this 2nd experment is of procedure of transformation
I.e. simple
Bacterial Transformation:
1. Pipette 200ul sample cells into each of 3 ice cold Eppendorf tubes. Label the tubes Control, 1 ng, and 10 ng (1 ng is 10 -3 ug, or 10 -9 milligrams). The unknown plasmid is at a concentration of 1 ng/ul. Add 1 ng of your unknown plasmid to one tube and 10 ng to the other. Place the tubes on ice for 30 min.
2. Put the tubes at 42oC for exactly 90 seconds. Return the cells to ice for 1-2 minutes.
3. Pipette the transformation mixtures onto labeled plates containing ampicillin and spread them around using a sterilized, bent glass rod spreader.
4. Place upside down in the 37oC incubator in room 305 overnight.
5. 16 - 20 hours later, count the number of colonies on the plate with well-isolated colonies. Put parafilm around the edge of a plate and put it in a refrigerator for later use. Check the control plate to see that no colonies grew on it.

And the viva voce, that was very simple :) I scored 10/10 :)

Okay, let us see some of the questions that examiner asked me.

1) Question: Which gene responsible for antibiotic resistance
Answer: some plsmid have Ability to make host cell resistance to one or more antibiotic and these also transfer the resistance to cell lack R factor i.e. resistance
Sub questi

2)   Question:  What is plasmid and give some examples
Answer: Plasmid is extracellular DNA in prokaryotic oraganism and some are artificially synthesis plasmid also its genetic material ,
There are several types of plasmid

   F plasmid - fertility plasmid
   R plasmid - resistance plasmid
   col plasmid
   Promiscuous plasmid
   Virulence plasmid

3) Tell me role of STET buffer ??

 sir actually it contains Sucrose which helps in maintaining osmotic pressure; Triton X which helps in cleaving the cell wall; EDTA which acts as a chelating agent; Tris HCl is used as buffer.

nice tell me ur roll no ?
sir it's 69 😊😊

  

Bye meet with new subject .....

Whats Your Favorite Dish

Hi, I am  Cooking for my little one my cute one and love them lot and I love u also

SO what's your favorite dish ?

Everyone has his favorite dish like plants love urea  phosphorus and minerals my garden need manure for my garden

I'm a bit different; I never grow roses,lilies, sunflowers in my garden; But, just cute little microscopic flowers

microbes!!! Each and every person will have their own favorite dish.

Mine is Tandur and butter masala. What's your's?

What do these little cells require for growing well? 
But, what do my microbes need? What's their favorite?
Got an answer!!! Got an answer!

They love Agar!!! Starting with basics of preparing nutrient agar in my lab tomorrow.
I use to ask some questions to my professor and some i search online

Oooooo... My microbes are nonveggy. They love beef extracts, so i must add some to my nutrient agar!

Got some questions :

1) At what temperature does agar liquefy and solidify???

        It's very simple to answer this,
Solidifies at 32 to 40 degree C.
Liquefies at 80 degree C.

2) What role does agar serve as a nutrient ???

         Most microbes can't destroy and feed up agar, it just gives a background for holding the nutrients and the microbes. They give support to the cell wall of certain algae! It's a polymer made of galactose units.

3) Why agar instead of gelatin ?

Gelatin liquefies at even less temperature in the range of 35 to 40 degree C. But, Agar melts at 80 degree Moreover, only few microbes can degrade it , hence it can provide a better solid surface for growing microbes.

4) Nutrient agar for my microbes with contents like :

Glucose ---> Provides carbon                    source.
  Peptone ---> Gives protein
  Beef extract ---> aminoacids, sugars, inorganic salts
  Nacl ---> For maintaining osmotic pressure.
  
  NaCl is generally added to the nutrient media for maintaining the Osmotic pressure. Maintaining osmotic pressure is important, because, increase or decrease in the osmotic pressure leads to cell burst or death due to the effect of osmosis. So, maintaining the osmotic pressure is done by adding correct amount of NaCl. ( I learn this concept in last semester I like to relete science concept in various experiment)

Got tired out of typing and reading my notes and papers. ooov.. Gonna sleep.. good night!!!
will feed you tomorrow my little ones.!!! :P

Suger A sweet villan

Its nice to write here after a long time, how are u all 😊
Nowadays, I'm very much interested in reading something new regarding my subject, "Biotechnology".People say, "oh Tahakik you are bright student, then why you chose Biotechnology?
It has no scope. ,😣😣
You won't earn better. You might have chosen Engineering , Pharmacy or medical , UPSC's.
something like that..." But, who cares 😐💰💰
I'm loving my subject. Biotechnology is the branch where you can survive only when you are much interested. anyway
I'm thirsty , reading a lot for my ongoing and new research project. But, I'm finding it sometimes much difficult to organize and start with. I'm getting hundreds of ideas, but, I find some of them silly, I find  some of them  complicated, I find  some of them  impossible! But, still, with hope in myself and interest in the subject, I'm continuing with them spend lots of time to think and playing with ideas ...
My dream is to  find something new and publish an article in a high impact journal.
Okay, let's stop here with my story, and go with the main matter. (sry for this )
We all love sweets, isn’t it? From a cute baby who had just started feeling and enjoying the tastes to 100 year old people, love sweets! 😘😋

Oh, I could hear you, yes, "Diabetic patients"
can’t enjoy sweets.
But, they too love to have sweets. Even, my Grandfather is diabetic and he uses to eat sweets hiding from my gramdmaa
Okay, what for that sweet love,🌷🌷
you are asking? Nothing wrong in that, but, if you are crazy about the bottled drinks, then, I’m sorry, you must start worrying!

Aspartame – is an artificial sweetener which is used in most of the bottled drinks. And, that is an approved artificial sweet nerby FDA (US Food and Drug Administration –which tests and approves all food and drug items before they are marketed)

“It is approved by FDA, then, why we must worry?”, is this your next question? Let me answer you!Aspartame is formed using two

aminoacids –
L-Phenyl alanine
L aspartate.

This when broken down during metabolism in ourbody produces – phenylalanine and hence must be avoided by people who are suffering from the genetic disorder called Phenyl Ketone Urea (PKU) – these people can’t metabolise phenyl alanine further!

This Aspartame will be unstable at higher temperatures and they are stable in the pH of 4 in general. As most of the bottled drinkshave the pH of 3 to 5, this is generally used in bottled drinks.(prefer chilled)

Moreover, this is preferred because this has 200% greater sweetness than the normal sucrose we use.

This is enough for us right?
The taste? The taste is enough, right?Okay, what is the problem in consuming thisaspartame? Only the PKU people must avoid this?

No, even normal healthy individual’s health will be affected on consuming aspartame in long run – many researches revealed this!

There are several published articles narrating the link between the aspartame consumption and Cancer! Yes, cancer,

leukemia!In 1965 Aspartame was discovered and was approved by FDA initially in 1974. Even after the approval, the safety of aspartame is being questioned by many researchers and the FDA considered the approval again!A trick to hide the risks behind a product is generally done by showing the results of short term tests and analysis. People are misled by such short term analysis!The metabolized products of aspartame not only include aspartate and phenyl alanine but also methanol. Methanol is toxic! But, many scientists and researches argue that methanol could be degraded by alcohol dehydrogenases. But, this degradation results in formaldehyde which will degrade DNA and proteins! This will lead to several Auto immune diseases and cancer!

And, researches reveal that men are affected more by the consumption of aspartame!
Many products like this, which are synthesized artificially might have long termeffects over our health, as this is going to affect us only in long run we won’t realize this.My mom use to advice me not to take bottled drinks and she suggests fresh juices instead (By Patanjali 😂😂).

She says, if I drink bottled items, it would affect my health.
It’s true isn’t it? Aspartame or other artificial sweeteners might be the reason behind this?I’m not sure about the worst effects of Aspartame, I just wrote this post by reading various articles and information already published.You are sick? May be it is due to artificial sweeteners? To check this, just avoid artificial sweeteners. Check the label of everything you consume sweet! If you are fine after avoiding these artificial sweets, then, the problem is with the artificial sweeteners.Just try to be safe with natural sweets!

ग्रुप्स आणि मी

प्रत्येकाला काही न काही अडचणी असतात. कोणी बोलतं, कोणी बोलत नाही. कोणी आपापले मार्ग आपणच शोधतात, कोणी इतरांना विचारतात.

पण ह्या सगळ्या प्रोसेस मधे सोबत कोणीतरी हवं असतं. नेहमी सगळा प्रॉब्लेम शेअर केला जाईल असही नाही. पण नुसतं बोलायला, व्हेंट आऊट व्हायला हवं असतं.

कधी माहिती - मदतहवी असते. या सगळ्यामधे प्रत्येकाच्या कॉंट्रीब्युशन असते, असावी लागते. कधी नुसतं एेकून घ्यायची, कधी सल्ले देण्याची,
*टर्न बाय टर्न हे प्रत्येकानं केलं तर गृप घट्ट होत जातो*

कधी एखादीला सतत माहितीच हवी, ती मात्र इतरांच्या मदतीला येत नाही, असं झालं की मग हा बंध सैल होऊ लागतो. सतत एकीलाच पँपर करत रहाणं हेही काही फार संयुक्तिक नाही.

तसच कधीतरी तुमची चूक दाखवली जाऊ शकते हेही लक्षात घ्यायला हवं.

तर कधी कोणीच मदतीला येऊ शकत नाही हेही समजून घेतलं पाहिजे.

प्रत्येकाचं मत वेगळं, अनुभव वेगळा, स्वभाव वेगळा,... हे प्रत्येकानेच समजून घ्यायला हवं.
आणि समजा एखाद्याला नाही समजलं एखाद वेळेस तर ते सोडूनही देता आलं पाहिजे.

सर्वात महत्वाचे म्हणजे गृप मधून बाहेर पडताना, नीट सांगून बाहेर पडायला हवं. परतीची वाट ओपन ठेवा.

हे सगळं लिहितोय ते खर तर माझं मलाच सांगतोय. सो नो ऑफेन्स प्लिज

इत्यादी...

तुला मिठीत घ्यायला
मला ' आवडणार ' नाही...
आवडत नाही असे कुणी सांगितले ?
तसे फार वेळा मनात येते
पण मग तिथे गोष्टी संपत नाहीत
मिठी संभाषणासारखी असते
आधी साधत नाही
मग कधी संपत नाही
आता त्या न संपणाऱ्या न घडणाऱ्या
संवादाचे काय करावे?
मिठी रेंगाळनार्या संवादात
'इत्यादी' असतोच ना
तसे तू मिठीचे करून टाकतेस
इत्यादी इत्यादी घुसमटनारे इत्यादी
दिघ:मूढ इत्यादी शब्द,स्पर्श,व्याकुळ ,
इत्यादी इत्यादींच्या अनेक तऱ्हां......
इत्यादींची मला भीती वाटते
तुलाही वाटते
तुझीही वाटते
इत्यादी भीती वाटण्यासाठीच असतात
किंवा निरर्थकमध्येच
केंव्हा तरी
माझ्याकडे वळणार्या
तुझ्या आतुर नजरेसारखी
ती निरर्थक असते !!!
असे नव्हे ,
पण कदाचीत तसेच असते
कारण ती इत्यादी असते
इत्यादी म्हणले तर अर्थपूर्ण असतात
म्हणले तर निरर्थक
मग कुणी म्हणले तर......
इत्यादी,इत्यादी........

(तहकीक ऋषिकेश रवींद्र)

BROKEN WORDS

कविता इथेच संपल्याचं 
मधेच कऴल्यावर 
पुढल्या सगळ्या ओळी 
खोडतांना
आठवत राहातात
अनेक संबंध 
ज्यांच्यातलं कवित्व संपलं होतं
कधीच पण
लिहित राहिलो होतो 
त्यांच्याअनावश्यक ओळी आपणच
अपरिहार्यपणे
कागदावरल्या ओळी 
कापता तरी आल्या 
पण संबंधांना तसंच ठेवलं तरंगत 
आपण निर्दयपणे 
वेगवेगळ्या कवितांच्या
गल्ल्यांतून चकवा लागल्यात
पुन्हा पुन्हा पोहोचलो 
त्याच हमरस्त्यांवर तरी संबंधांवरली शीर्षकं कायमची खोडता नाही आली
आपल्यालाआणि चांगल्या ओळी 
वेगळ्या काढून 
संबंधही Re write करता आले नाही 
अज़ून नशेत लिहिलेल्या
कित्येक ओळिंचे संदर्भदुसर्या दिवशी लागता लागले नाहीत 
किंवा खपली खालच्या साकळल्या 
ओळीही भळभळल्या नाहित 
कधीच तरी संबंधांचे 
व्रण मात्र कायमचे उठतच राहिले आपल्यावरआता
कधीतरी 
आज़वर कापलेल्या ओळी 
आणि 'उगाच' वाढलेल्या संबंधांना 
"मांज़रीचं" गोंडस् पिल्लू 
समज़ून अंधार्या पठारावर यायला हवं सोडून

आपापल्या पायांवर 
त्यांनी स्वताःच 
शेवटी मोठं व्हायला हवं म्हणून....!!

Lowry Assay Principle and procedure

Good evening all of you its nice to write hear my lab experience i.e. now become habbit to write this to me and now my  aim is to make perfect my practical skill because to hone perfect my overall grade points. Now i stop hear blowing my own trumpet and start for topic
Thought there are several protein assays available,we do DNS last time but  the most preferred one in many laboratories is "Lowry assay". It is effective in the concentration range of 0.1 mg/ml to 1 mg/ml.(we use 1 mg per ml i.e. add 50 u in 50 ml water )
As  per procedure provide And, as an additional info, the paper published describing the procedure and principle of Lowry Assay is the most cited paper in the scientific history. (Feeling like, "Wow! I want to publish one to compete with Oliver.H. Lowry"???  :P) (i goohled this )

Why Lowry?
Though there are several other protein assays, mostly Lowry assay is used in many laboratories. The reasons for preferring Lowry are: sensitivity of the assay, highly reproducible, simple and easy .

Principle behind Lowry's Assay for protein with procedure
The reactions that occur in Lowry assay are binding of Copper to the Nitrogen in the peptide. And, the phosphomolybdic tungstic acid in the Folin Ciocalteau reagent gets reduced to hetero poly-molybdenum blue by the copper catalyzed oxidation of aromatic amino acids in the peptide, in alkaline conditions. The assay must be done at the pH of 10 to 10.5 as it is sensitive to pH changes.

Reagents Required:

A) 2% of sodium Carbonate (50 ml) + 0.1 N NaOH solution (50 ml)
B) 10 ml of 1.56 CuSo4 + 10 ml of 2.37% Sodium potassium tartarate

Lowry's Reagent = 2 ml of (B) + 100 ml of (A)
Folin's Reagent what we use in our laboratory is ready made one (2 N) which is just diluted and made as 1 N for use. (by mixing with equal volume of water)

For constructing Standard curve, BSA is generally used. Stock of 1 mg/ml is required.

Note: Prepare all the reagents in distilled water. And, prepare the reagents freshly before use, just before use, to avoid precipitation of the salt added, also mix the reagents A and B only before use.

Procedure:
First the BSA stock is diluted for standard curve construction. take 50 ml in 50 u BSA stock and
Then make sample o.1 mg per 1 ml of distilled water.
Then took 7 test tube mark them
B, 1,2,3,4,5, uk
Similarly, we can prepare various standard concentrations like 0,0.2,0.4,0.6,0.8,and add salivary amylase in unknown 1ml for  total volume of 1 ml by mixing 0.1, 0.2,0.4,0.6,0.8,1 ml of stock BSA with 0.9,0.8,0.6,0.4,0.2,0 ml of distilled water respectively.
From this prepared standard concentrations of BSA, 0.2 ml must be taken for assay. For example, from the 1 ml of 0.05 mg/ml BSA prepared 0.2 ml must be taken in a separate test tube. Similarly, 0.2 ml must be taken from all the other standards.
Then, 2 ml of Lowry's reagent must be added to each of this 0.2 ml sample and incubated for 10 minutes.
Then, 0.5 ml of Folin's reagent is added to each of the incubated tubes and incubated for 30 minutes.
After 30 minutes, the tubes will have blue colored solution, which is further read at  660 nm in Spectrophotometer.
Graph should be plotted with absorbance in y axis and concentration in X axis.
And, using this standard graph, we can determine the concentration of unknown sample by extrapolation.

This is a very easy assay which I learnt in the firest year.

You know, I feel the major disadvantage of this Lowry assay is that you need to spend at least two hours to complete it including reagent preparation and incubation. (Sometimes, I feel like, oh, no, incubation for 30 minutes!How good it would be if there is no need for incubation?) And, when you don't have that much time,
And after this we go to show results of optical density in calorimeter to sir ,sir amaze to see results( i thought results may
Incorrect because there may mistake in stock a d working solution we took 1mg per 1ml sample ) while thinking this sir say why this division results are correct we dont have answer if this but i get answer of my doubt that we can use BSA solution @ 0.1 mg per 1ml to 1 mg per ml both results may correct..

Hope, this helped you :) Found any mistakes? Doubts? Let me know with your messages

Caffeine-How much did u need ?

Now a days, coffee comes in many varieties, such as Nescafe - Rubusta  and Bru- Gold , as well as different flavors and sizes.
Colas are made with artificial flavors and added caffeine. The amount of caffeine in all of these different drinks can vary widely. Coffee used to be just "black". Coca-Cola was originally made with Kola nut extracts and contained cocaine ??
no wonder it was so popular! Energy drinks are a new trend in highly caffeinated beverages. They contain a wealth of sugar and other natural stimulants that help provide that sought-after energy boost. Caffeine is also found in many weight loss preparations and in some over-the-counter pain, diet and stimulant medications.

Here are the most common sources of caffeine In India

Coffee - Contains about 100mg per 250 ml. cup though most coffee .

Black Tea - Contains 50mg per 250 ml Cup

Green Tea  -contains 25mg.

Caffeinated Sodas - Coke, Pepsi, and others contain 50-60 mg per 500 ml

Super-Caffeinated Colas -

contains 80 mg per 500 ml can.

Energy Drinks - Red Bull and monster contain about 80mg per 8 ounce can.

Milk Chocolate - Contains 6 mg/ounce.

Medications - Anacin contains 32mg/tablet. Extra Strength Excedrin contains 65mg/tablet.

(Source PTI **)
I googled this and got this information okay this is not important what this numbers say i. e. important,

How much do you consume? Add it up and see. I would guess, if you are a typical caffeine Lover, that you top out over 300mg per day.

Caffeine has long been considered an unhealthy lifestyle choice. Caffeine's negative effects on the nervous system and how it increases anxiety, heart rate and sleepless nights have always been a concern. But recently coffee and caffeine have been shown to have some significant medical benefits.
I recently read a article in Nature Scientific Journal regarding caffeine i.e. mentioned that
After more than 10,000 scientific studies over the past 30 years the findings indicate that people who drink one to three cups of coffee a day are less likely to contract diabetes, develop Parkinson's disease, or have gallstones. Additionally, coffee reduces the risk of colon cancer and cirrhosis of the liver. Some of these findings may be due to the health benefits of the coffee bean itself, but most can be linked directly to caffeine. Coffee has also been shown to be beneficial in asthma (caffeine is a bronchodilator), stopping headaches (a vasoconstrictor) and improving mood (releases dopamine), all due to the systemic actions of caffeine.

In spite of these beneficial effects, it is still recommended to consume caffeine in moderation. Enjoy your day cup(s) of joe - or my favorite, Diet Pepsi - but remember that too much of a good thing is not always better. Everything in moderation, nothing to excess.

First Few days in MGM-CABT

It was 20th june I believe! I just saw the details of the Maharashtra agriculture research institutions(MCAER) undergraduate programs only a week before the last date. I decided to apply for it and just applied in a hurry actually, yes, it was really a hurry! I was done with everything and sent the application.

I was waiting for the release of selected list of candidates from the 25th july They updated the selection list everyday but my name was not on the list till 25th of March!that time i require admission is very essential otherwise my 1 year may spoil or if i took wrong decision may my whole carrier in danger to i remain very cool try to encourage myself because i fail pmt with narrow margin i dont want to took admission for BAMS or pharmacy . I used to go to the MCAER website everyday and check the list, but, my name was not there in the selected list of candidates. I lost all my hope and decided that I won't get selected. But, it happened! I received a mail from the Academy after 29th of july! I was happy to the core, jumping up and down. Because, after the 3 month of negative stress there is something to makes me happy from school time my favorite subject is biology its felling a good i go for most advanced course of natural science in no1 college

I believed that this would change my destiny to a better place! The college is not more long than my town but i required change

I was happy till my father said "no" to this. He was really afraid to send me out of my town! But, I managed to convince my dad and he accepted to allow me to take mebin hostel

And, the next problem after convincing my dad was, "when to join for the mgm cabt After lots of confusions, finally joined mgm hostel gandheli which was allotted me @ 7th and college starts 8th August

And, this is the first time for me, away from home!

Boys hostel. My room mate, he is a vaibhav kale and I'm really enjoying her company.

mgm college agri Biotechnology
In the college  I got some new friends firest day lectures by mr. Chandre and mr shukre both of them goes basics in Biotechnology i was found active in class because we disscuss my fav subject i.e. Scope for biotechnology this day was play imp role in my image build up i.e. Day scholar student..

I now aware of all tensions forgot all problems in gandheli and its makes i love gandheli

Even i begain again for makes poems, write articles that i stops in cet period i.e. Very stressful time i got only 140/200 marks

After some days i

Introduced with B.N. Chavan sir who is principal and dean of our college they tell us about degree programe rules and regulation of college

The normal college timing is from 9.30 a.m to 5.oo p.m. And students do research even late nights which shows their ultimate interest. People here are dedicated!

Learning many things apart from the academics, which i hope will be very useful for the rest of my life. It is very safe in here and the place is really "God's own Country". People here are very friendly, smiling and caring, so that getting acquainted with the new environment becomes very easy!

Finally, I'm feeling free and independent for the first time in my life with a complete satisfaction of what I'm doing right now!
Many of the memories but i share after some day i go for completion practical the exam on head !

best wishes......

Yours friend
Rushikesh

All I Needed to Know I Learned in ... ???

I know I am going off on a tangent here, away from my destination of a Creationist's view of biotechnology

but I was thinking that there are many books and essays and teachings in the secular world that men use to guide their lives that either contain lessons similar to those in the Word or are directly or indirectly based on them. Three such books to me are:

All I Really Need To Know I Read  in These Books  it is the "secret "
and
The Seven Habits of Highly Effective People by Stephen R. Covey

Each has several key directives to focus on to make your life more relational, less anxious and guided by a purpose or destiny. Lets take a closer look at some of the directives from each book.

All You Really Need to Know

All I Really Need To Know secret is a book of  Rhonda Byrne , and book become so popular until it get in my hand,
The title explain itself what is book actually about i.e. lists lessons normally learned in everyday life and some secret of great people  they  explains how the world would be better if we all lived by the same rules; sharing, being kind to one another, cleaning up after ourselves, and living "a balanced life" of work, play, and learning. The book contains over 50 short Lesson, reflecting on topics ranging from love,relationship,life,and social life

Here are a few of the points Rhonda Byrne

makes in his opening essay:
Live a balanced life
Clean up your own mess
Play fair
Share everything
Here are some  that direct us to a similar path:

Live a balanced life - learn some and think some and draw and paint and sing and dance and play and work every day some.
- There is a time for everything, and a season for every activity under heaven:
- Learn to do right! Seek justice, encourage the oppressed. Defend the cause of the fatherless, plead the case of the widow.
- Finally, brothers, whatever is true, whatever is noble, whatever is right, whatever is pure, whatever is lovely, whatever is admirable--if anything is excellent or praiseworthy--think about such things.
Clean up your own mess - Clean up after yourself.
- "And when he comes, he finds [his house] swept and put in order.
- Let all things be done decently and in order.
-  And the second [commandment] is like it: "Love your neighbor as yourself."
In a subsequent blog we will look at the next book,

"The Seven Habits of Highly Effective People"

Breathing My love

Beginning......(Written My first science paper)

I recently published my first research paper in International Journal of research with IMPACT factor (5.6) not only my first in any journal, but also my first as a sole or lead author. I have never written an article for a scientific journal before, though I have experience of scribbling , here in this Blog, 

Now, I know enough about how to to write a research article, How to handle research experiment,it's so exciting and fun to learn new things, I've done lots of mistake and learn so many new things also , till before i am not aware all this and  Today I am writing my first Research experience as a UG student here we go....
Yes there are long list of peoples who help me to sucess in writeing a research paper My professor, my juniors my sweet and cute classmate's, & my dear besties, Some FB and Whatsapp frds, and all my wellwishers thank  you very much all.... 
The path from idea to publication was long, winding and sometimes wandering off in the wrong direction i was so excited and it makes some mistake in manuscript writing
Like many teachers, my interests in Biochemistry education research are firmly rooted in classroom practice rather than in the rich, pedagogical literature.
The activity that I presented in the paper actually came from Learning a lectures from my BIOCHEMISTRY teacher. Sir was explaining us about Spectrophotometetry and I imagining about Is it possible to measure Phosynthetic activity by this method how cool it is just by extracting leaf sample we measure Phosynthetic activity but sir also explain that only biomolecules can quantity using this technique. and I just go for it I have read lots of books regarding this but I never get ant idea then I am go for check Oxygen amount by calculate oxygen amount i.e. one of byproduct of photosynthesis and by calculate oxygen amount i.e. easy to measure Phosynthetic activity I just go for it I done lots of experiment most of them go fail but every failures i got something new and I finally   After nearly 2 month of practising the activity in my college laboratory, and sharing it with my colleague  I thought I was onto something that was worth luckily communicating.Choosing somewhere to publish was tricky
I wanted a peer-reviewed journal with a good impact factor to support my fledgling academic career, but I’m not happy with educational content being behind a paywall. However, the best match for my work was the Journal of Agricultural Education, which unfortunately has just that issue.
Internal conflicts aside, I then set about embedding the work in the existing literature. Strictly speaking, this is the reverse of the ideal, more efficient research process. The process of ‘referencifying’ felt very much like a literature review exercise that I might set for undergraduates. I buried myself in papers on teaching sequences and student misconceptions until I had enough of a framework to put my work in context.
I called in favours with My professor and my friends who are established researchers and already publish their paper in High IMPACT journal,asked them to give me feedback.
This act build base of my research paper , I redrafted mistakes and correct typographical errors, add references, read more paper's, and make new abstract rewrite result and conclusion, i try my best to make paper according to their comments
I finally submitted the paper to International Journal of Research and after 3-4 days they repLy on my E-mail that they like my paper and they Resend me. research manuscript for minor revision then I pay publishing fees and they ready to publish my paper in March issue so blessed 
That’s a 1 month gap, so what happened in the meantime?
The administration side seemed to run reasonably well and I had comments back from reviewers. It was only then I realised that my Professor  Chandre sir had shielded me from the real brutality of the peer review process.
The three reviewers fell with almost comedic placement into
(a) it’s great, publish it as it is,
(b) it’s ok, tweak it and send it back and
(c) The concept for the paper is fine and could be a useful contribution. Thereare spelling mistakes in the manuscript which should be corrected. More result-oriented discussion may be added. Also more justification of results against simulation work may be added further. Overall the paper is accepted with minor revision.
Some of the comments were cutting, suggesting there was nothing new about what I was reporting and querying why I hadn’t referenced publications in journals that were so obscure even our (extensive) university library didn’t have access to them.
It was hard to reconcile the comments of reviewer (c) with those of reviewer (a), and it felt very personal. Some kind words and email counselling from those same experienced friends helped me to regroup and take reviewer (b) as the middle ground and work from there.
So the paper was rewritten with a clearer emphasis. I took advice from an Mr. Mahesh chandre and Dr.Ashwinikumar kshirsagar to help decode some ‘lost in translation’ moments which had come across in the reviewers comments
A final hurdle: the images I had provided (taken with the camera on my Galaxy Note 3) but still they were low quality to publish (I am not a good photographer it's proved). This, farcically, led to me retaking and providing much higher resolution images, which were too large to be processed by the online submission system.
What I learned: lessons for anyone new to publishing education research
My research interests have now developed into new areas but I don’t think I will completely lose the tendency to do things backwards, Learning  will probably always lead to ideas for me.
I would start reviewing the literature earlier in the development of the idea, even if I had to rely on Google Scholar rather than waiting for better access to journals.
I would remember that reviewer comments, especially in large fields like education, are rarely personal, and I would laugh off the most absurd comments.
I would also sort out my side of the administration earlier; copyright forms and, of course, image requirements
In the end, the value in writing a research paper is why we should be encouraged from the start to approach it as it was intended to be approached ...Find my paper here