Thursday, 8 November 2018

Book Reading | The Gene: An Intimate History

"This book is the story of the birth, growth, and future of one of the most powerful and dangerous ideas in the history of science: the "gene," the fundamental unit of heredity, and the basic unit of all biological information. I"

~Siddhartha Mukherjee


There can be nothing better than reading books but it becomes quite a luxury when we add the time constraint. Having oversubscribed myself to quite a good number of magazines, I find it hard to commit myself for a book.
However, the other day while browsing through the non-fiction section of the Public Library I stumbled upon the second book by Siddhartha Mukherjee; The Gene: An Intimate History. 

The author has mathematically noted down each milestone in genetic science in this book, rather descriptively. But with all this, the author ensures that his readers do not lose their focus from the fact that study of genes is not anymore just a progressive or evolutionary aspect in science and medicines, rather it is a study and observation about how an individual is insufficiently deciphering the technological advancement in the science of genetics as well as the study of oneself.

I had always longed to read the first and Pulitzer winning book by this author - The Emperor of Maladies. So when I saw ‘The Gene’ available on the rack I couldn’t avoid grasping it. The book is voluminous but still I decided to take up the challenge. And I have been doing pretty good at that.

It would be futile attempt for a person like me who last read biology in 10th standard to write an academic review of the book. But I would rather limit myself to writing why this book is a worthy read for a person who loves science.
‘The Gene: An Intimate History’ as the name suggests tracks historically one of most important discoveries of science right from the ancient times, when it was just as concept to the ancient thinkers and philosophers, to the modern day unraveling of the mysteries related to the unit of heredity.
I have always had a liking for history but the history of science is something we get very seldom to read. We have well documented history of society, politics, war but science lacks its share. What we get of science history are only the first one or two pages at the beginning of text-books which are written as part of the introduction. We always miss the point that it is important to know the history behind a scientific discovery or invention to understand its importance. It is also important to know the society and politics of the time and then we will understand the weight-age of what Galileo, Copernicus, Darwin or others had achieved.

Knowing the history of science is necessary to understand the painstaking efforts put by scientists to arrive at the final objective. As the author discussed in this book how Mendel toiled for 8 years just growing peas in his garden and conducting the experiment which statistically or theoretically established the presence of a unit of heredity. That experiment or the results thereof may seem quite trivial in context of the recent advances made in the field. But appreciating the scale of the achievement is important for a student to understand how big an effort is needed to arrive at small conclusions.

Reading the book would help a reader from non-life science background to get answers quite a few curious questions they may have. What is gene? What is DNA? How is gene different from DNA? Where do you find the gene? How does gene carry traits? The reader would be amazed to read how a chemistry transforms into life form. It would also seem that at a very basic level human being is nothing but an aggregation of chemical compounds and chemical reactions taking place at minuscule levels.

Reading the book I tend to ask the question where is the soul? Is soul nothing but proteins which are the driving blocks inside the human body? Reading the book, it almost seems that expanse of the universe is encoded in the expense of the DNA.

The historical parallels presented by the author are equally fascinating. We may have read how the Bolsheviks overthrew the Tsar but how gene originating from the lineage of Queen Victoria of England had to play a role in that is hard to fathom and find mentioned in any book on history of the revolutions.

The book goes into technicalities quite often but then the author does a good job to bring it back in sync with broader theme of Gene’s history.

These are type of books which should be included as reference material with school curriculum. Sometimes just studying science without knowing the applications and implications it has led to becomes quite monotonous. Reading such books as this one would help establish everlasting love with subject.

Sunday, 5 August 2018

An Friendship Day

I hope you are doing well..
…….

We don’t choose our parents but we have full on freedom to choose our friends. Yes!! Friends are our extended family. The one who tells you the truth and never breaks your trust is your true friend and he or she don’t make you feel inferior but you feel more strong because of their support.
Sometimes friends have the hidden intention. They may later turn into enemies because of something.

We all have lots of stories on the topic which is friendship the relation and bond we create with the person with whom we have similar sort off wavelength. Friends are like rare precious gems one can’t find them easily.
Well!! You can find a friend like in any age group and from any stream. He or she just knows you so well….that person will be the one with whom you share your joy and sorrow…!

We all know every relationship is built on the base called as trust.

These days we have experienced that everyone have become more realistic and is lacking the personal interaction. And this may affect our mindset about friendship…
Friendship shall not be influenced by money and all stuffs.

It doesn’t matter how much wealth you have but you shall have good friends…that also matters!!

Some of the people don’t prefer being with friends as they find being with them as a waste of time. There are people who don’t move an inch if their friend don’t come at their place…there are so many!! Whatever case friendship counts… And you are gonna have that one person who will mean a lot to you.. The one you will call for your birthday, the party… Wedding… This and that.. Coffee meet ups…. So many events!! So much to speak about.

There are friends who will be with you only for a month or so but the bond you will share will be unbreakable.. Yes!! Such friendship exists…!!

There could be friendship between a boy and girl.. Of course!!
Having a girl to be the bestie is the blessing…really!!
Look all boys don’t love to talk all the time about  Politics and gossiping stuffs.. But they love things such as play and stuffs like that… Those type of Boys are more happier when with Girls… No no not for their attention.. But because they can be playful and enjoy…

Friendship takes lots of effort..to maintain!!
But when you try with all your heart in.. It’s smooth sailing and doesn’t take much hard work..

Your teenage period and when in your twenties and thirties.. Your behavior and thoughts are influenced by the company you are with.. Friends!! That’s why it’s said.. Man is known by the company he keeps!!

Elders keep us telling that make friends with Care. When we are in school… Parents make sure that they ask our Buddies grades… It happened with all of us..!!

People make assumptions that if one is friend with someone from Rich background can create trouble for the one who’s from middle class… No no.. Friends don’t have class… It’s just there!!

For some people dogs are their best friend and for some it’s all about having good books.. Books are their best friend..
See!! Friendship doesn’t have a shape or things like that.. It’s totally up-to us!! Totally!!

Make your life a party by making good friends…

Do yourself a favour… Choose good friends!!

A song from the movie Sholay says a lot about how the bond is.
Tera yaar hoon main depicts how a friend always wants best from you..
listen at this song too..

If you are among those who’s trust was broken by friends.. Or haven’t found the true friend yet… You too be loyal…

Let’s be friends  …

So!! Happy Friendship Day!!

{ Be your own best friend too }

Take care!!!

๐Ÿ˜Š๐Ÿ˜Š๐Ÿ˜Š

Saturday, 21 July 2018

The last page of my notebook...

A place where I check 

whether my pen is working or not

A place where I calculate 

the percentage out of the marks I got


The last page of my notebook...


A place where I play the game 

of flames with my secret crush's name

Then strike it off so that no one sees

and not giving a chance for my best friend to tease


The last page of my notebook...


A place where I chat

with my friend when the class is going on

Unable to listen 

the boring lecture going on since morn


The last page of my notebook...


A place where I note 

important questions for the upcoming exam

A place where I scribble 

random things or even try drawing the funny face of Ma'am


The last page of my notebook...


A place where I write 

beautiful lines of my favorite song

A place where me and my friend play 

tic-tac-toe with marks of right and wrong


The last pages of our notebooks... 

aren't just pages, They are much more... 

They are precious diaries...

where I unknowingly treasure our teenage memories..

Tuesday, 29 May 2018

Yess !! I write poem's

Many people write poetry today!
I also thought that writing poetry was a big trick.

Now I'm writing poetry, my favorite poem. This is a kind of poet that I have not really tried so far.

Okay, I'm going to write poetry, what poem? What is the theme of the poem? Do you think of all this?

Let me tell you, this poem I write now is very sweet. My favorite is my friend.

Yes, this is the poem that I write for my girl-friend. She's always the game. Laughing. Now, even though she thinks, her smile looks forward.

" Supports you, okay ?

Now you can write " kitti bitti mitti " the language that she speaks to, and enjoyed my language too!

"Let's write fast ... " , I was saying this, I started talking to her, " Kidu kittunu write "
I began to say this.

The fact is that we are going to change the way we work with him. But no matter who we are, we do not change.

For example, if you do not like someone, if you do not like his character, you will not be with him.
If we take a person's character we will start with him.

Some cuisine may be wrong with our prediction, so there is a chance of friendship.

If you do not like this one then it is difficult to be friendly with him.

For some time, we will fight with hatred, but we can not be separated from them for a long time.

We know that he is our favorite despite fighting.

My favorite person is my friend. Every day we both talk a long time.

I'm lamenting, she's lamenting to her. I cry with me once I cried!
Whenever you feel angry with my father at home, your father, mother, will say peace.

I do not know if everyone is like this sweet friend!

When we seek to do evil, our true friendship will prevent us. Thank you for having such a friendship.

However, I, I ... do not saddle too much with someone. Even though I am a friend, I will keep a distance. I have never walked with my friends, like everyone else! Not at all. The mind does not always give me a place to talk to me as I can not talk.

The pain is causing me. Sit down and talk to your friend for hours, but do not bend the lap to the bed!

Does the mind not give it, why? I do not know!

I do not always feel sorry for anyone who knows how to speak with smile.

Poem, this poem is about this. My friend, my friendship and habit is about this.

poem. It's poetry, now you've read poem.

Do not rig, it's poetry, in fact it's not poetry! Kavitha, my friend Kavitha.

Did you say the above? This is a record for her, that she would speak with a smile and say a kid. For my poem!

Do you think this is a wild moksha? Think of it, but it's my poet, though it's mocking you. That's enough for me!

Monday, 28 May 2018

I'm telling you!

It's not a letter, no poem, but it's just sure it's for you!

The relationship between heaven and earth is only by you and me, yes, from the distance, it looks like it's bundled, but never been together!

You know, the star and the moon do not know where the distance is, and you can be enjoying it, but what to do, is not the moon all day, just like our friendship, Many time.

It's you!

After sleeping, wake up in the middle of the night and call you and talk to the laugh and dream of everything I've dreamed of, but, bar, even the story that is nothing is sweet. You and me!

Your favorite song and my favorite song, and after four hours of change, we do not get bored and we continue to chat even five hours.

My favorite activities, how did you find it, talk to me and talk to me in just one hour? What can I easily imagine my mind?

"You're so sweet," I'm saying the word word nowadays. What is the use of The only time you've got, the only thing you've got to find is the only thing left! Today it seems to me that it's too late for you and me to talk to me and to speak on that day!

"What's up" is what you say. You are going to take everything that I do.You sin, what are you doing, no other way? You do all the things I'm doing all the time! I have not said this before, and now I say, "You and all the lords that you are making," she said!

"I will not talk today." I will tell you it's easy, I'm sorry, how is your face going to be heard?

I know that, when you finish reading it, run to me and say beautifully, "Look at me".

I'm waiting for that! Where are you going to say ?

To run away


A little breathing, pulling and breathe once, another time, was a little lazy. Do not think of anything, no, no! I think that's the way I think of it and that's only left! What is that? It's a matter of necessity here. No need to tell.

Something grieving, was you yesterday? There was no last week? No, right now As well as a grievance.

If anyone can say, "This is not a big grievance," says this knowledge.

Always this struggle, "Let's be this, it's going to be a sort of nervous thing, have you done this?"

Since it is the one that has already predicted that it will happen, "It's already anticipated?"

What if you do not have trouble? What is that mind? Knowledge? What's the difference?

Here's how to make me run everyday, "Do something, show me that you are bigger, run, run, and here you will appreciate if you do that, hand it, run over ...", so what makes me run that way? Shall? Yes. That's it. It seems to see everything as an object, a praise and a success. It makes me run to do one thing. Planning and executing the project.

Every time a single landing becomes active, success is more likely, more likely to fail, and correctly, sometimes, yes, exactly.

The tile runs to run.

Manaco? Sin is not willing to run it, Wait, how much of the coconut tree and the flower is beautiful. In the run up to this day, sin is endowed with the effect of all the work that knowledge is to celebrate the success and defeat of failure.

It's a little relaxing, it's a failure or a defeat, after both you have a little rest.

Know or not, it's time to run, run over, run away.

Two years ago, after my house, the mother planted a little bit of a little tree with water everyday.

The roses of my father in the pot in front of the house are blooming!

Baba, who was born a year ago in the house floor, has two pretty teeth.

The paint house is changing "a pink coloring" to the opposite house.

There is a neem tree on the way, two coconuts are missing.

There are so many changes that have not seen anything in the fast run!

This announcement is like what today, after a long time, you can not run, do not run, say, stop and wait for a few days.

Changes in the flow are all the above. Aunt, can not even see the opposite house running properly ???

The truth is, early in the morning, when the house was closed after the evenings, it seemed unnoticed.

"Do you have a little time and walk around the house and do you know for a while?"

"Have you ever walked on a fast bike" sir ", the street of my town, can you walk on it?"

"Can you once again go to the street and play on the street?"

The mind is like to ask all these things, want to ...

"Lusa you? What can you say about the neighborhood, the big booze, the little boy in the street? "

Is it all the same?

Talking to women .... how?


"It's a mistake to talk to a man and a woman" "No man and girl can be friendly!" All this is always a grandmother in my town. But what is that? Grandma is great, if he says right? Is not it?

My mother would say, "It's a thousand words mean bigger!" But, "Is it wrong to talk ???", which are all justifications? Now, if you say "speaking wrong", you say, "

Rushi you're like that person."

Speaking of male and female today, the friendship is a simple thing! At school, in college, at work, there is a need to talk and talk to "everybody". But it's just how we talk about what the other thinks about us!

Some people will be laughing and laughing, even when they see. Some of them will ask for a loan to speak a word.

When most of the first segments have been caught by everyone, but "retarded" often, they will fail and speak well, take the place and spoil the impression on him.

Whatever you talk to, you can talk very well, but there should be a gap in the middle. If it is specifically talking to women ...?

It's good to talk a lot! Here are some tips, how do you talk to a girl? Some tips for If you follow this, do not you have (damnation?)

Yes ... our daughter is a girl, daddy ... this guy ... said the feet powder, where the guy is not a sin?), Ayya ​​.. ", that is not like feel!

Most often, women will talk a lot, but they will speak well, but this is a problem!

"Daa, she does not talk to me ..", she went to town and hit the end, and finally the lady talked to her in the limelight of love! So first of all, let's not think of a girl talking to her!

Just go and do something like a great achievement! Even if you love the girl, do not talk about the girl talking like this story! It will come to know the girl anyway, even if you have a good impression on you, powder!
Then there will be some people, the first time you come up and come straight and talk like

"Hello Baby .. Hi Bobby."

If all these things speak, it will not work! And especially for our women!
Is a girl working with you? Is a girl

studying Talking about study, studying, or working on a job can often talk friendly, but as I said before, it is good to have a break.

"What are you talking about? Well, friends, if you want to tease, talk, but it's a bit more fun to be a girlfriend!

And it depends on every girl, some women tease and talk too much anger, some people do not like clean. So, when you start talking first, there is always a space to leave.

If the days are good friends, then if the lady does not take a big deal of kidding then, okay!
Too much, "buy, go", is special to talk. If that girl is a very good girl, if she does not like talking to her, she will say, "Why do you buy this?" If you are a younger than you, talk to a girl who is a bigger girl. However, if you often speak to a college student at school, "buy it.", It will be somewhat different. But it is good to talk! The lady has a good reputation for you! If you're not talking to a studying girl, "go shopping," it's good to talk to a new woman who does not know, "go shopping!"

And then you do not have to forget it, since it is common to see who you look at, Just look and talk with a little smile, do not laugh, "EEE" like "Raja Rani Arya"!
Some people will, if they like a girl, go back and bother to irritate them! Come talk back to iPad! Some are, "are you single?" , "do you have a boy friend?" They know that, if they say "single" they think they can try! Please do not trouble a girl to please all this. If you ask, you will not be impressed by most of them!
There are some people who are working with a girl or a student who has a mobile number and no time to call them at any time to call them ten eleven o'clock in the night! If it's a serious matter, it's okay, it's okay. It's all right to avoid this!

Then there will be some people, the marriage will be over, "The fight with the wife, the problem in the house is the life of my life," she said to her, Is this fine? Is it not good for a good man to complain about working with his wife?
"Vady, Podi ..", do not forget! Respect is important. A similar age, or even younger than you, "come on", is enough!
Do not talk without reason. Speak for any reason. If you want to talk without a need, talk only if you are a very good friend, and do not talk too much about the first time when you speak the first one!
Most of the above-mentioned things apply to all women. But for some time, if you already have a lot of people or you have a great deal of respect for the girl you're talking about, how can you accept it? But it's harder to go if you're getting more trouble or take advantage of "advantage".

If you have to say a little bit, talk to a girl, enough space!

"If the gap, let's talk a six feet, six feet away ???" If it came, "I tell you the truth, I can not explain to you!" :) :)

Talk right!

Saturday, 26 May 2018

เค…เคชुเคฐ्เคฃเคค्เคต

आज खूप दिवसांनी लिहायला बसलोय तेही पहाटे 5 वाजता, नुकतच दुर्गाजय दत्ता च पुस्तक वाचलं "The Boy Who Loved" वेगळंच  वाटतंय...एकदम रोमॅन्टिक ! सर्व भूमिका आणि चरित्राची अगदी सुबोध वर्णन केलंय अगदी हळवं झालंय मन, खुप भावना दाटुन आल्यात वर पाऊस टीप टीप पडतोय वातावरणात एक गारवा आणि पहाटेची किलबिल मोहक शांतता यासोबतीला उन्मुक्त अभिव्यक्ती. सडेतोड आत्मपरीक्षण..वेदनांची हळुवार उकल.. आणि समोरच्याच्या साथीने आपल्या स्वतःलाच सापडलेले आपण! फार सुंदर अनुभव होता, खरंतर कुणा दुसऱ्यासाठी घेतलेलं हे पुस्तक इतकं आवडेल अस वाटलं देखील नव्हतं , आता लक्षात येतंय किती हद्दपार केल्यात काही गोष्टी आपल्यातून. ‘माझ्या’ माणसाला मनातलं काही सांगताना मोबाईलचा स्क्रीन वर टिपटिप करण्यात नुसताच वेग साधतो.
धावणारे विचार काबूत आणून कागदावर उतरवंताना मजेदार तारांबळ उडते, ती सरावाने ‘प्रोग्राम्ड’ मेंदू अगदी सहज उरकून टाकतो. हरवलेला आहे प्रत्यक्ष स्पर्श. ‘पर्सनल टच’.आपल्या प्रिय व्यक्तीची भेट कधी होईल माहिती नाही. भेट काय, तिचं दर्शनही अप्राप्य आहे. पण संवाद तर साधायचाच आहे 'या मनीचे त्या मनी' घातले नाही, तर ते अपूर्णत्व आपल्याला चरितं करणार आहे. ही सैरभैर करणारी ओढ. कधीकधी अपूर्ण गोष्टी असतात ना, त्या भासतात. पूर्णत्वाचं समाधान नसेलही त्यांच्यात कदाचित. पण अर्ध्यात वेगळ्या झालेल्या त्या वाटा धुक्यासारख्या भासतात. गूढ..पण सुंदर.😊

Saturday, 7 April 2018

Dancing Young Girl


I saw her dancing so I stayed,
unable to depart,
Because of talents there displayed that stirred my very heart...

The moment that she stopped was when I ventured learning more,
As if new courage prompted then as I had crossed the floor...

She looked ahead and saw me smile and in that moment knew,
That very soon and in a while, I'd whisper,     'I like you! '😊
And And she smiled beautifully, as if to coax me on,
I flirted quite outrageously with all my doubts now gone...

And then I took her hand to dance, a slow dance cheek-to-cheek,
With that sweetheart I'd met by chance, so precious and unique...

I'm meeting her tomorrow night, our next dance as agreed,
A second chance to get things right, because this time I'll lead !

Wednesday, 7 February 2018

Happy Valentine's Day ; Don't Forgot sweet's for your sweetheart

Hiiiii.......
Reader's

It feels so good to write here, after a long (so long, in fact) gap. How are you all doing? I’m doing great, did you know, I'm near about Final year of my course
since I wrote a post here. And now, got time to share with you.
me trying to keep me busy with books for Competitive exams like ICAR-AGRIL, JNU like university for  M.Tech (Biotechnology)
This is time for valentine search ! For the valentine's day, we youngster's use to celebrate this day like a festival Many ppl around us  celebrate Valentine's Day by showing appreciation for the people they love or adore. Some people take their loved ones for a romantic dinner at a restaurant or Give chocolate ;
Most all of us love the taste of chocolate, you may find lucky if u got such gift on such special occasion ;  but for some it means someone may get hurt! no need to get hurt we can buy and enjoy from any shop (like me do always)

Chocolate is a traditional Valentine's Day gift, in a heart shaped box, or in a heart shaped candy, wrapped in red foil.

"All you need is love. But a little chocolate now and then doesn't hurt."
I goggle this thought ; anyway it's not important what it means that important

Chocolate comes from the seed of the tropical Theobroma cacao tree. Cacao originate in Mexico,and distributed  Central and South America, in india also northern state cultivate chocolate,
chocolate is fermented product,
The seeds of the cacao tree have an bitter taste, and fermented to develop the full flavor of chocolate.

Much like coffee beans, the cocao beans are then dried, roasted, and ground to form cocoa mass, a pure chocolate called chocolate liquor. The liquor contains two principle ingredients: cocoa solids and cocoa butter. Cocoa solids contain theobromine, a methylxanthine alkaloid chemically similar to caffeine with like physiological effects.

There are numerous types of chocolate made by mixing four ingredients,

cocoa solids,
cocoa butter,
sugar, and milk

solids in varying proportions. Additional agents such as lecithin (an emulsifier), vanilla and vegetable oils increase the possible formulations. Here are some of the most common forms used:

Unsweetened chocolate: Also known as “bitter” or “baking” chocolate. It is unadulterated chocolate: the pure, ground, roasted cocoa beans rendering a strong, deep chocolate flavor.

Bittersweet chocolate: A dark chocolate that contains at least 35% cocoa solids. Most bittersweet bars contain at least 50% chocolate liquor, with some bars pushing 70-80% chocolate liquor. This chocolate has a deeper, more bitter flavor than sweet dark or semi-sweet chocolate.

Semi-sweet chocolate: A dark chocolate containing at least 35% cocoa solids but is sweeter than bittersweet due to additional sugar.

Sweet dark chocolate: A “dark chocolate” as it does not contain milk solids, but with a high percentage of sugar so it is much sweeter than other types of dark chocolate. Many brands of sweet dark chocolate have only 20-40% cocoa solids.

Milk chocolate: In addition to containing cocoa butter and chocolate liquor, milk chocolate contains either condensed milk or dry milk solids. Milk chocolate must contain at least 10% chocolate liquor, 3% butterfat, and 12% milk solids. Milk chocolates are typically much sweeter than dark chocolate, and have a lighter color.

So I hope you did not forget your sweetheart this Valentine's Day and bought her a box of her favorite health food, Chocolate!

Saturday, 13 January 2018

Diagnose your disease!


Let it be any disease, ranging from a fever (which is the symptom of many diseases) to HIV, diagnosis at an early stage is very important for giving specific treatment.Whenever I go to a doctor, he asks me things like, stomach pain, fever, body pain, head ache, running nose, throat pain etc., then, he checks pulse, then he uses his stethoscope, he then gives all the possible antibiotics! Yes, really, this happens in most cases. Then, if my problem is not fixing up in a week, he'll ask me to take a blood test, this, that, and, all!

Nothing wrong in this, because he can't ask everyone coming to him to take blood test at the very first sitting, then, no one will visit him back saying he sucks out blood all the time! But, it is okay to have a blood test after a week, but, not after a month! Diagnosing a disease after it had reached a severe stage is comparatively less significant than diagnosing at an early stage. So, how to diagnose?

Antigen - Antibody!
Antigen and Antibody interactions are mainly used for diagnosing most of the diseases. When you give your blood sample to your doctor, he gives it to his lab, there they check for the presence of particular antigen in your blood. For example, if you have typhoid, surface proteins of  the causative organism (Salmonella typhi) will act as antigen and when antibody (specific protein produced by your body against the antigen) is added to the infected blood sample antibody - antigen interaction occurs, which could be detected by various techniques.

Possible methods for diagnosing a disease!
There are several methods available for diagnosing a disease, but, most of them are based on the antigen-antibody interaction. Here I had listed a few methods of diagnosis, which I know:

1) ELISA  - Enzyme Linked Immunosorbent Assay!
This method is based on the antigen antibody interaction. Two antibodies are used for detecting the presence of a single antigen. But, both the antibodies won't be directly reacting with the antigen, one antibody (primary) will be reacting with the antigen, and other antibody(secondary -which was raised against the primary antibody) will react further with the primary antibody used, producing color based on the enzyme linked with it. Generally done using 96 wells plate.

2) Western Blot!
This is also done for finding the presence of a specific protein (in diagnosis, the protein is antigen) in a given sample.This is done generally after doing SDS PAGE and transferring the protein separated in the gel to nitrocellulose membrane followed by similar technique of adding primary and secondary antibody, which will show color if there is infection.

3) PCR - Polymerase Chain Reaction
Though the presence of a particular gene could be detected using Southern blot, the most sensitive technique used for diagnosing a disease based on genes is PCR. Quantitative PCR could be done for even finding the severity of the disease based on the concentration of a particular gene of the causative organism.

PCR

I prefer to go with PCR than any other technique, but, when there is no thermo cycler in your laboratory it's better to go with ELISA! There are also other techniques like latex agglutination and microscopic observation of samples, but, the best way is PCR!

What do you say? PCR?

เคœเค—ाเคฒा เคถेเคคी เคถिเค•เคตเคฃाเคฐा เคฆुเคฐ्เคฒเค•्เคทिเคค เคจाเคฏเค• : เคกॉ.เคชांเคกुเคฐंเค— เค–ाเคจเค–ोเคœे

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