Breathing My love

Beginning......(Written My first science paper)

I recently published my first research paper in International Journal of research with IMPACT factor (5.6) not only my first in any journal, but also my first as a sole or lead author. I have never written an article for a scientific journal before, though I have experience of scribbling , here in this Blog, 

Now, I know enough about how to to write a research article, How to handle research experiment,it's so exciting and fun to learn new things, I've done lots of mistake and learn so many new things also , till before i am not aware all this and  Today I am writing my first Research experience as a UG student here we go....
Yes there are long list of peoples who help me to sucess in writeing a research paper My professor, my juniors my sweet and cute classmate's, & my dear besties, Some FB and Whatsapp frds, and all my wellwishers thank  you very much all.... 
The path from idea to publication was long, winding and sometimes wandering off in the wrong direction i was so excited and it makes some mistake in manuscript writing
Like many teachers, my interests in Biochemistry education research are firmly rooted in classroom practice rather than in the rich, pedagogical literature.
The activity that I presented in the paper actually came from Learning a lectures from my BIOCHEMISTRY teacher. Sir was explaining us about Spectrophotometetry and I imagining about Is it possible to measure Phosynthetic activity by this method how cool it is just by extracting leaf sample we measure Phosynthetic activity but sir also explain that only biomolecules can quantity using this technique. and I just go for it I have read lots of books regarding this but I never get ant idea then I am go for check Oxygen amount by calculate oxygen amount i.e. one of byproduct of photosynthesis and by calculate oxygen amount i.e. easy to measure Phosynthetic activity I just go for it I done lots of experiment most of them go fail but every failures i got something new and I finally   After nearly 2 month of practising the activity in my college laboratory, and sharing it with my colleague  I thought I was onto something that was worth luckily communicating.Choosing somewhere to publish was tricky
I wanted a peer-reviewed journal with a good impact factor to support my fledgling academic career, but I’m not happy with educational content being behind a paywall. However, the best match for my work was the Journal of Agricultural Education, which unfortunately has just that issue.
Internal conflicts aside, I then set about embedding the work in the existing literature. Strictly speaking, this is the reverse of the ideal, more efficient research process. The process of ‘referencifying’ felt very much like a literature review exercise that I might set for undergraduates. I buried myself in papers on teaching sequences and student misconceptions until I had enough of a framework to put my work in context.
I called in favours with My professor and my friends who are established researchers and already publish their paper in High IMPACT journal,asked them to give me feedback.
This act build base of my research paper , I redrafted mistakes and correct typographical errors, add references, read more paper's, and make new abstract rewrite result and conclusion, i try my best to make paper according to their comments
I finally submitted the paper to International Journal of Research and after 3-4 days they repLy on my E-mail that they like my paper and they Resend me. research manuscript for minor revision then I pay publishing fees and they ready to publish my paper in March issue so blessed 
That’s a 1 month gap, so what happened in the meantime?
The administration side seemed to run reasonably well and I had comments back from reviewers. It was only then I realised that my Professor  Chandre sir had shielded me from the real brutality of the peer review process.
The three reviewers fell with almost comedic placement into
(a) it’s great, publish it as it is,
(b) it’s ok, tweak it and send it back and
(c) The concept for the paper is fine and could be a useful contribution. Thereare spelling mistakes in the manuscript which should be corrected. More result-oriented discussion may be added. Also more justification of results against simulation work may be added further. Overall the paper is accepted with minor revision.
Some of the comments were cutting, suggesting there was nothing new about what I was reporting and querying why I hadn’t referenced publications in journals that were so obscure even our (extensive) university library didn’t have access to them.
It was hard to reconcile the comments of reviewer (c) with those of reviewer (a), and it felt very personal. Some kind words and email counselling from those same experienced friends helped me to regroup and take reviewer (b) as the middle ground and work from there.
So the paper was rewritten with a clearer emphasis. I took advice from an Mr. Mahesh chandre and Dr.Ashwinikumar kshirsagar to help decode some ‘lost in translation’ moments which had come across in the reviewers comments
A final hurdle: the images I had provided (taken with the camera on my Galaxy Note 3) but still they were low quality to publish (I am not a good photographer it's proved). This, farcically, led to me retaking and providing much higher resolution images, which were too large to be processed by the online submission system.
What I learned: lessons for anyone new to publishing education research
My research interests have now developed into new areas but I don’t think I will completely lose the tendency to do things backwards, Learning  will probably always lead to ideas for me.
I would start reviewing the literature earlier in the development of the idea, even if I had to rely on Google Scholar rather than waiting for better access to journals.
I would remember that reviewer comments, especially in large fields like education, are rarely personal, and I would laugh off the most absurd comments.
I would also sort out my side of the administration earlier; copyright forms and, of course, image requirements
In the end, the value in writing a research paper is why we should be encouraged from the start to approach it as it was intended to be approached ...Find my paper here

STUDY Holidays

Study Holidays

Hello everone Glad to write here after a long time !!!
How are you all doing? I’m doing great, did you know, I am little busy nowadays and trying to express myself And, after 20 years of life under the warmth of my parent’s wings, I am thinking that  finally i should have to managed to spread my own wings,and i am doing same

I was little busy in my own world, Enjoying holidays,writeing research paper, Business plans ,Attending seminar and National level conference 3 times,Writeing for my blog, dating,writeing for Our newspaper, college magazine and many more in this semester, Make hone and perfect my skills

This is a personal post. I can say I am sharing my mind voice with you But did u know our exam schedule on 13th march and I started studying by today
You can find my way of learning, my preparations for my semester exam.

I'm in the end of my study holidays!(What i do in my study holidays

What I do during study holidays?

1) Reading New Books,Autobiography of great people's ,
2) Eating snacks and enjoying T.V.
3) Sleeping in afternoon
4) Went to exhibition two times
5) Writing stories, poems
6) Helping dad in their work

These pleasant days, is over now My Theory exams were scheduled on 27th April, and I started studying on 1st April . I started completing Mannual @100 WPM

I m, I started playing with out preparing for the exam! I started sitting like a ghost nowadays! Yes, I play all day, and at the end of the day I start reading for my exam by 6 or 7p.m.
And, Yesterday I am starting with Techniques in Biochemistry exam , I played Cards :P :D , but, I never win the game

Started studying by 6p.m had dinner in between and studied non stop till the next day 1 am ! Late night study's
I know, this is not good for health, I need a good sleep. But... I don't wanna miss the fun at home during the day time by sitting alone and studying. My little brother and  friends, they all are enjoying their school summer holidays. They are making fun and much noise during day, then, how come I can read?

I'l take my book, pen, notes and start reading, they'l start shouting, "Rushidaa shoot shoot.. come on.. hey.." looking at their computer games! That's it, I'l close my book, jump over to games :D :)  enjoying the beauty of my day!

Towards the end of the day, they all go home, I'l sit with my books! I started spending my nights hugging my books instead of pillows. Anyways, I manage to prepare well. Let's discuss my question paper now!

1. Principal method of separation of biomolecules ?

Biomolecules are extracted from mixture of biomolecules they have widely dissimilar physical and chemical properties i.e. molecular weight,solublity,charge,molecular size pigment ect. and hence easy to isolate them from mixture

Centrifugation
crystallization
Decantation
Evaporation
Distillation
Electrophoresis
Filtration
Sublimation
Chromatography

Question.2

Types of Chromatography

A.Plane
  i)Paper
       a) Ascending
       b) Descending
ii)Thin layer

B.column

Molecular drive
ion exchange
affinity
gas liquid
HPLC
Gel permission

Question 3.
Spectrophotometetry

Question 4. PCR

Question 5.  Principal behind centrifugation

Question .
DNA microarray
DNA microarray are the recent development for DNA sequence and screening genes ,the DNA probes are immobilized at define position on solid surface made from Nylon or glass the probes may be DNA Molecules or C-DNA,or synthetic oligonucleotides.

ABOUT ME

Introduction ?

 

okay let me introduce myself ...

This is all about what i am Agricultural biotechnology student from MGM CABT,Aurangabad, Maharashtra

I love learning and especially biology and I like to continouing basics in biology with biotechnology

my ultimate AIM is utilise my knowledge which i receive from my masters degree so firest of all i have to complete my masters degree in any CENTERAL GOVT. institute and makes hone perfect my practical skills by work with an biotech industry and in future develop my own biotech industry and I hope i will make something that would add value in humans life

I am not a genious, and not a average i am last bencher but not think i am poor in acedimics i m oneamoung topper in my class i always try to came out answers when prof. addressed a question my hand always raised with yesss ??
I cant remain  quite in my class i always asking dought about lecture and doing lecture with 100 concentration

i am not narrate all this for blowing my own trumpet to impress this for write my days of learning ''my learning journey" this for realize you that you can except good stuff from me

i i like to share my lab experience mistake rectification exam doubt so i ve open an whatsapp group for this
i read know understand and faught for answer still when i not get a proper concept
so ive know enough  about my subject
so anyone can except useful from me i love my teachers i am a keen observer ,
also i enjoy to inspire people

there may be mistake because i share the things only i read understand and i.e.possible  sometime i may wrong to understand complex concepts
to fell free to show my mistake and help me to clear my own doubt too
my favourate subject are cell biology molecular biology microbiology biochemestry
loads lots of honey and enjoy sweetness of biology

तू गझल आहेस

सोज्वळ सावळ्या रंगात नटलेली
तू एक गझल आहेस
वाचताक्षणीच मनात पोचलेली
तू एक गझल आहेस

प्रसन्न विचारांसारखे केस आहेत मुक्त
हासिले गझल असे ते ओठ स्मित युक्त

हवी होतीस तेव्हा भेटलेली
दिसताच वसलेली
मनाला भावलेली
तू एक गझल आहेस
अर्थांचे अनेक झालर असलेली
तू एक गझल आहेस

डोळ्यात तुझ्या चमक आहे आत्मविश्वासाची
वृत्तीत तुझ्या लकब आहे जग जिंकायाची

मराठमोळा बाज असलेली
तू एक गझल आहेस
कितेक युगांनी  सुचलेली
तू एक गझल आहेस

The Mind Crunch Mimamsa : स्त्री, एक परिचय

The Mind Crunch Mimamsa : स्त्री, एक परिचय:                              पहन ली वो ढीली सी कमीज और पायजामा। अपने बेतरतीब बड़े बालो को छोटे छोटे काट नकार दिया मैंने लड़की होना। अचानक स...

वाडा चिरेबंदी

वाडा चिरेबंदी .....

वाडा चिरेबंदी,
तसा आजूनही आहे उभा

आब-रुबाब उरला नाही
घोडे-तांगे बारदाना गेला
कळा गेली ,रया आली
घोशा सुध्धा उघडा झाला

वाडा चिरेबंदी
तसा आजूनही आहे उभा

शेतं आताशा पिकत नाहीत
बळदं -पेवं भरत नाहीत
फार कशाला वर्ष भर
दाण्याला दाणा लागत नाही

वाडा चिरेबंदी
तसा आजूनही आहे उभा

रोजगार हमीवर सारा गाव
रोजच कामासाठी जातो
शे-पन्नास रुपये रोज
हमखास कमावतो
वाड्यातील लोकांना मात्र
बुलंद दरवाजा आडवा येतो

वाडा चिरेबंदी
तसा आजूनही आहे उभा

पावसाळ्यात गावातील
घरांची पडझड झाली
सरकारकडून गावाला
नुकसान भरपाई मिळाली
वाडा मात्र पडला नाही
तेव्हा त्यांनी त्याला
लाखोली वाहिली

वाडा चिरेबंदी
तसा आजूनही आहे उभा

नोकऱ्या मागायला गेले तेव्हा
"पाटीलकी" आडवी आली
टेबला खालून आले हात
तर खिसे होते खाली
आज दुश्मन झाली वाड्याला
वाड्याचीच सावली

वाडा चिरेबंदी
तसा आजूनही आहे उभा

एक दिवस पेपरच्या
फ्रंट पेजवर बातमी आली
अमुक तमुक गावात
सामुहिक आत्महत्या
एक कुटुंब उधवस्त

वाडा चिरे बंदी
तसा आजूनही आहे उभा

आता तेथे सार्वजनिक
धान्याचे कोठार साठले आहे ......
  कवी- रवींद्र तहकीक

Rio Olympic

ऑलिम्पिकचं हे थीम साँग. केटी  पेरीनं गायलेलं. ते ऐकतानाच एक सळसळता उत्साह संचारतो
काय नाही या गाण्यात?
झुंजण्याची चिवट जिद्द आहे,
कितीही छाटून टाकल्या फांद्या
तरी मुळापासून रुजण्याची आणि पुन्हा पुन्हा फुटण्याची आस आहे..
आणि स्वत:वरचा असीम विश्वास आहे.
जो म्हणतोच आहे, डोण्ट डाउट इट! डोण्ट डाउट इट!
कारण जिंकणं हे माझ्या नसानसात भिनलेलं आहे.
आणि त्या जिंकण्यासाठी मला कुठलीही तडजोड मान्य नाही.
कसलीही लाचारी मंजूरच नाही.
मी संघर्ष करीन, अफाट संघर्ष करीन
स्वत:ला अंतर्बाह्य बदलून टाकीन
आणि मी जिंकेनच!!
**
हे गाणं खेळाडूंना उद्देशून असलं तरी ते गाणं फक्त खेळाडूंचं नाही. ते आपल्यासारख्या प्रत्येक सामान्य मनाचं गीत असावं, जे म्हणतंच आहे की,
या अफाट गुणवत्तेच्या स्पर्धेत
कसंबसं टिकून राहणं मला मंजूर नाही.
मी सर्वस्व पणाला लावीन जिंकण्यासाठी, जिंकवण्यासाठी!
पण हे सारं करताना मी भानावर राहीन!
स्पर्धेतली ईर्षा, सगळा मॅडनेस, कल्लोळ
या साऱ्यातही मी भानावर राहीन!
त्यामुळे माझ्या जिंकण्यानं आश्चर्यचकित होऊ नका,
मी जिंकण्यासाठीच आलोय!
मी पुन्हा पुन्हा उगवेन, पुन्हा पुन्हा संघर्ष करेन
आणि पुन्हा पुन्हा जिंकेन!
कारण जिंकणं हे तर माझ्या नसानसांत
रक्तासारखं वाहतंय!
***
रिओ ऑॅलिम्पिकपचं हे थीम सॉँग. ते येत्या महिनाभर जगभर गाजत राहील.. आणि एकच संदेश देत राहील,
जिंकण्यावर, मेहनतीवर विश्वास ठेवा,
पायाशी आगीचे लोळ उठले,
आकाशात गिधाडं घरट्या घालत असले,
कुणी तुमचे लचके तोडू पाहत असलं
तरी तुम्ही संघर्ष करा..
कारण जिंकणं,
आणि जिंकणंच फक्त तुमच्या रक्तात आहे!

I won't just survive
Oh, you will see me thrive
Can't write my story
I'm beyond the archetype
I won't just conform
No matter how you shake my core
Cause my roots, they run deep, oh

Oh, ye of so little faith
Don't doubt it, don't doubt it
Victory is in my veins
I know it, I know it
And I will not negotiate
I'll fight it, I'll fight it
I will transform

When, when the fire's at my feet again
And the vultures all start circling
They're whispering, "you're out of time."
But still, I rise
This is no mistake, no accident
When you think the final nail is in; think again
Don't be surprised, I will still rise

I must stay conscious
Through the madness and chaos
So I call on my angels
They say...

Oh, ye of so little faith
Don't doubt it, don't doubt it
Victory is in your veins
You know it, you know it
And you will not negotiate
Just fight it, just fight it
And be transformed

When, when the fire's at my feet again
And the vultures all start circling
They're whispering, "you're out of time."
But still, I rise
This is no mistake, no accident
When you think the final nail is in; think again
Don't be surprised, I will still rise

Don't doubt it, don't doubt
Oh, oh, oh, oh
You know it, you know it
Still rise
Just fight it, just fight it
Don't be surprised, I will still rise

Quantification of DNA

Quantification of DNA!
Hi,
I explained the principle behind the extraction of pDNA in my last post. Extracted the DNA, what next?
Let us quantify the DNA extracted.

How to quantify? Very simple, all you need is Spectrophotometer.
Just take 1 microliter of your DNA extract in an eppendorf, make it upto 500 microliter with nanopure water. (else 1000, or any desired volume)
mix it well
Now, put this into a quartz cuvette (0.5ml), measure the O.D value at 260 nm. Use blank as nano pure water.
Note: The path length of the cuvette must be 1cm.
Okay, we got the O.D value now, how to get the concentration of your DNA?
It's very simple, do the following calculation.

If your O.D. Value is 1, then, the concentration of your DNA sample is 50 microgram/ml

so, now, if your concentration = obtained O.D.( let it be 0.5) * 50 microgram/ml * Dilution factor

How to calculate the dilution factor?
Simple! here your dilution factor is 500/1 i.e., 500 micro liter contains 1 micro liter of DNA, you can calculate according to your dilution.

Now, using this calculation you can get the concentration of your DNA sample! Very simple, isn't it?

Principle:

DNA has absorption maximum at 260 nm; that's why  we are taking the O.D value at 260 nm. This absorption maxima at 260 is due to the presence of nitrogenous bases in DNA.

Proteins usually have the absorption maxima at 280 nm.

DNA is hyperchromic - i.e., the O.D value increases with denaturation of DNA. i.e, single stranded DNA absorbs more than double stranded DNA.

How to check the quality of your DNA sample?

Take the O.D value at 260 nm as well as 280nm .

Calculate O.D 260 / OD 280.

If it is equal to 2, then your sample is 100% pure!
If you get values below 2, there is contamination with protein.

Understood? Any doubts? Feel free to question me!

Thanks, Bye, Wish you a happy time!

दोष नाही

शाप होता चांदण्याला तो ढगांचा दोष नाही,
वेळ होती ओहटीची सागराचा दोष नाही.

छेडल्या तारा तरीही सूर नाही वाजले,
का फुलाच्या लाजण्याने हात माझे भाजले.
हात होते छेडणारे तो फुलाचा दोष नाही.
शाप होता चांदण्याला तो ढगांचा दोष नाही II१II

आरशांचा खेळ होता; सावल्यांचे हासणे,
दूध माझ्या भावनांचे साखरेने नासणे.
भूल होती; भास होता; भावनांचा दोष नाही.
शाप होता चांदण्याला तो ढगांचा दोष नाही II२II

चूक होते प्रश्न ते की चूक होती वेळ का?,
पावलांचा चालण्याशी बैसला ना मेळ का?
पावलांची चूक होती चालण्याचा दोष नाही.
शाप होता चांदण्याला तो ढगांचा दोष नाही II३II

बांधुनी डोळे कसा गं घेतला तू फैसला;
रेशमाशी गुंतताना गुंफला मी कोसला.
हा किड्याचा कोष होता रेशमाचा दोष नाही.
शाप होता चांदण्याला तो ढगांचा दोष नाही II४II

वेदनांच्या पावसाने आज माती भाजली;
दोन डोळ्यांना तुझ्या गं आसवे मी पाजली.
दोष नाही आज माझा हा तुझा ही दोष नाही.
शाप होता चांदण्याला तो ढगांचा दोष नाही II५II

एक सुंदर संध्याकाळ

ना जाणे क्यु ....... 

संध्याकाळची वेळ, काम करून करून कंटाळा आलेला, थोडासा fresh व्हावं म्हणून गरमागरम चहा चा cup घेऊन खिडकीत आलो. बाहेर काळेकुट्ट ढग दाटून आलेले. आता थोड्याच वेळात पाऊस पडेल हे सांगायला वेधशाळेची गरज नव्हती. काळ्याकुट्ट ढगांच्या साम्राज्याने अस्ताला जाणारा सूर्यही झाकोळला. त्या ढगांची वर आकाशात झालेली गर्दी उदासीनता आणून गेली आणि मनात अनेक विचारांनी गर्दी केली. विचार सहाजीकच “ती” च्या बद्दलचे.

काय विचार? विचार कसले प्रश्नच नुसते.. काय करत असेल ती? ती पण अत्ता चहा घेत अशीच खिडकीत बसली असेल का? तिकडेही असच वातावरण असेल का? असे एक न अनेक प्रश्न. पण का विचार करतंय मन या सगळ्याचा? काय उपयोग आहे याचा? पण उत्तरं कुठेच नाहीत. फक्त प्रश्न. विचारांच्या तंद्रीत चहा चा cup तसाच हातात. इतक्यात पाउस सुरु होतो. दुपारच्या उन्हात तापालेल्या धरतीवर पावसाचे थंडगार थेंब पडतात आणि धरतीमातेला एकदम शांत करून जातात. ओल्या मातीचा सुगंध साऱ्या वातावरणात पसरतो. त्या सुगंधाने मन थोडं हलकं होतं पण मनातलं वादळ काही क्षमत नाही.

इतक्यात  व्हाट्सअँप आयकॉन वाजतो आणि मनात चाललेल्या विचारांची शृंखला तुटते. आत्ता कोणाचा message असेल?
“ती”चाच तर नसेल ना? आणि काय आश्चर्य, “ती”चाच message. Message ऐकला  आणि सगळा mood च बदलला. पाहिल्या पावसानंतर वाळलेल्या झाडांना जशी नवी पालवी फुटते तसाच काहीसं. मनावर दाटलेलं मळभ एकदम क्षणार्धात नाहीसं झालं. असं होतं तरी काय त्या मेसेज मध्ये?

तर २२ सेकंदाच  गाणं  एक ऑडिओ  क्लिप 

 ''ना  जाणे  क्यु  होता  है  जिंदगी के साथ,

 ना अचानक  ये  मन , किसी  के  जाणे  के  बाद ,

करे  फार उसकी  याद 

छोटी  छोटी  सी  बात ...

 ना जाणे क्यू  ... "

नवल ,म्हणजे  हे  गीत  तिने  स्वतःच्या  आवाजात  रेकॉर्ड  करून पाठवलं  होत ,

 एखाद्या जादुगाराने जादूची कांडी फिरवावी ना तशीच जादू झाली काहीतरी. मन अपोआप शांत झालं. एव्हाना पाऊसही थांबलेला. आकाशातला काळ्या ढगांचा साम्राज्य अस्ताला गेलं आणि मावळत्या सूर्याच्या तांबूस प्रकाशात सुंदर असं इंद्रधनुष्य सायंकाळची शोभा वाढवू लागलं. जणू शांत झालेल्या मनाचं प्रतिबिंबच आकाशाच्या canvas वर उमटलं.

अझुनही हातात चहा चा cup तसाच होता. चहा एकदम गारेगार झालेला. एरव्ही असा चहा प्यायची इच्छाही झाली नसती पण आज मात्र जादूच अशी झालेली की गारढोण झालेला चहाही अमृतासारखा गोड लागू लागला.आणि  तीच  क्लिप  मी परत  परत  ऐकू लागलो  एका मेसेज ने किती मोठा बदल घडवून आणला. किती वेडं असतं माणसाचं मन. फार शुल्लक गोष्टींवरून दुखी होतं तर तशाच शुल्लक गोष्टींवरून खुश पण. फार अपेक्षा नसतात मनाच्या, पण माणसं अशाच छोट्या अपेक्षांकडे दुर्लक्ष करतात आणि मग निर्माण होतो दुरावा. अशीच एक शुल्लक अपेक्षा पूर्ण झाली आणि सगळा मूड च बदलला.

हातातला चहा चा cup रिकामा झाला आणि कामाची आठवण आली. आकाशाच्या canvas वर इंद्रधनुच्या सुंदर रंगछटा रेखाटून सूर्यही अस्ताला गेलेला. मग मीही उठलो आणि कामाकडे परतलो..!!!

तळटीप १: पूर्वी मी अशीच एक पोस्ट लिहिली तेव्हा अनेकांनी मला “ती” बद्दल विचारलं. त्यांना मला एक  dialog ऐकवावासा वाटतोय, “एखादी गोष्ट / कविता लिहिताना ते सगळा अनुभावावंच लागतं असं काही  नाही, काही गोष्टी कल्पनेतूनही अवतरतात.”