Reason Why U read this blog ...

Good Morning ladies and gentleman
I have started this blog as a fresh start. A fresh start for me to be the more positive, stronger me that I have become over the past several years. I am 20, in a happy and single life and appearing B.sc agriculture biotechnology , and I Damm passionate towards my graduation
I am student, writer, blogger, I love singing, and singer ,books and author's, l I like readings , I can read for hours, I can talk for hours, I love whatever I do 😁

I am at my ultimate happiness high and I wish for you all to feel the same. The future is a fabulous thing which you can sculpt to fit you so don’t be scared of the future, embrace it.

This Blog is for you.

Whatever you want me to write about I will, to my best ability.
I am no guru(teacher) and I give terrible advice but I will always try. I am just a normal boy completed just teenage on the brink of creating a brand new life for herself. In a way, we’re all doing that everyday, creating a new life each day as we plod along. I am giving a conscious effort to make myself a new life, however.

I am keeping active (walking, doing Pilates, swimming gym on occasion etc.), trying to speak to new people, making this group  eating healthy (at least trying) and keeping positive in my mind. I am me and that’s okay. In fact, that’s more than okay, it’s fabulous! And it’s fabulous that you are you! Don’t let anyone tell you different, ever.

You may find this Blog all very cringey but that is not my intention. I want to embrace you to love yourself. I will equally love you all (unless you’re very mean to me, I may love you less then). I am not here to push you, hell I hardly push myself! I am just here as a helping hand to tell you that you’re going to be okay no matter what. I can be your friend, your mentor or just some of  you don’t care about. It’s up to you. You are the author of your own story.

HITLER AND BIOTECHNOLOGY

HITLER AND BIOTECHNOLOGY- Ultimate killing machine 

Nazi scientists secretly researched the possibility of dropping malaria-infected mosquitoes behind enemy lines during World War Two, a German academic has claimed

Hitler’s scientists planned to use malaria-infected mosquitoes as biological weapons by sending them behind enemy lines in the Second World War, according to research.

SS leader Heinrich Himmler ordered secret research into how insects infected with the disease could be kept alive long enough to be used against the allies, German academic Dr Klaus Reinhardt has claimed.

In January 1942, the SS chief created a special laboratory at Dachau concentration camp with the official aim of finding new remedies against diseases transmitted by lice and other insects, as well as the typhoid that plagued German troops.

But in the journal Endeavour, Reinhardt said records kept by Dachau Entomological Institute revealed its scientists also pursued research into biological warfare.

They tested the life spans of different mosquito breeds to try and find one that would remain alive long enough to be dropped into enemy territory.

At the end of the trials in 1944 a breed of anopheles mosquito, particularly effective in transmitting malaria to humans, was recommended by the director of the institute, according to Reinhardt, from Germany's Tuebingen University.

Image Source : INTERNET 

It is not known whether there was a connection between the work of the Entomological Institute and the chilling experiments carried out by Dr Claus Schilling who used prisoners as human guinea pigs deliberately infecting them with malaria.

Schilling was sentenced to death by hanging after the war.

The mosquito research had to remain secret because Germany, alongside the allied nations, had signed up to the 1925 Geneva protocol banning the use of biological and chemical weapons.

But Reinhardt dismissed the project as "a bizarre mix of Himmler's smattering of scientific knowledge, personal paranoia, an esoteric world view, and genuine concerns about his SS troops" which proved of no use to Hitler’s war effort

MEMORIES

Since childhood I am passionate about natural science. I grew up in a village surrounded by forests with many different animals and plants where my parents were the accomplices of my discoveries, transmitting their passion for observing and understanding nature. When it came time to decide on my academic orientation, I did not hesitate at any time to specialize me in the field of Biotechnology.

Biotechnology is, as a traditional definition, “the technique that allows the application of biology to make a profit”. Actually, all applied biology can be defined as Biotechnology, placing its origin in the technique of making beer in ancient Mesopotamia. The new vaccines, treatments or stem cell gene therapy, are Biotechnology. Transgenic crops or production of insulin for diabetics, are Biotechnology. Currently, the development of new biotechnology, thanks to genetic engineering, is so great that it has come to be called “the science of the future”.

Traditionally, Biotechnology is divided in five colors, each one corresponding to an area of ​​application:

• Red Biotechnology, applied to medicine. Stem cells or gene therapy are clear examples of Red Biotechnology.
• White Biotechnology, applied to industry. This is the case of the production of insulin for diabetics.
• Green Biotechnology, applied to agriculture, as in the case of GM crops or new pest control systems.
• Gray Biotechnology, applied to environmental protection, a good example is the bacteria used to degrade oil.
• Blue Biotechnology, an obsolete term for marine applications of Biotechnology.

Biotechnology is an exciting universe that mixes in a surprising way mysteries and certitudes. Studying Biotechnology Grade I became aware of the importance of science and began to look around me in a different light… And very quickly I became passionate for Biotechnology! It cleared me the way to life and general scientific knowledge (Physics of biological processes, General Chemistry, Organic Chemistry…) and then delving into some areas of biology (Genetics, Microbiology, Biochemistry …) and finally treating biotechnology issues (Agricultural Biotechnology, Applied Microbiology …).

During my career I had the opportunity to meet with renowned researchers and professionals who contributed to making me a professional excited by science.

Currently I have the good fortune to work in this field and I am very proud to have dedicated my career to several projects that will changed my life. It is a privilege to work on what you like and what you studied. Also, it is essential for me to continue increasing my creativity and improving my knowledge to follow the technological advances of science. Like many professionals of nowadays, leaving the routine and freshened up towards a new direction

Thank you

Tahakik rushi

What I learn ....

This is all about "what I learn!" I'm a B.sc. agri. (Biotechnology) student from Marathawada krushi universityand  MGM

college of ag.Biotechnology, gandheli, aurangabad, maharashtra , India. I love learning and I'm happy with learning. Still continuing with the basics of biotechnology; I'am not a genius; I read, I know something about my subject; So, you do can expect something useful from me! I love my teachers; I'm a keen observer, so you can expect some good stuffs for learning from me. If you are a starter in the course of biology/biotechnology/in general life science, who enjoys fun, who is eager to learn, you would love me!!!!

I'm a last bencher in class, but, don't think that i'm poor in my academics, I'm one among the toppers of my class. And, I'm the one who always try to come out with answers when the professor addresses some questions. I love teaching my own classmates, Chatter box...! I use to be the first to say "yes", when the teacher asks for taking a practical. The reason for blowing my own trumpet is to give you hope that you could expect some useful stuffs from me :) :) :D :P
i would like to share my personal experience , what I feel, what I love!
I'm sharing my lab experiences, mistakes, rectifications here, because it might help someone who search for any doubts in biotechnology like basics, simple silly and useful doubts. I have lots of useful stuffs for bio learning over here.

There may be mistakes/errors because I share only the things I understood (sometimes, I may go wrong in understanding certain complex things) But, I take much care to provide error free material (up to my knowledge)

Feel free to read, Feel free to contact me for very basic biotech doubts. And one more thing, my favorite papers are "Microbiology", "cell biology", "Molecular biology", "Genetic Engineering", "Immunology", "Plant biotechnology", "Animal Biotechnology". I love microbes, horticulture, agronomy, .

Loads of honey for you...!!! Enjoy tasting the sweetness of Learning Biotechnology!
Cheers!! :D :)

On writting - Monsters are real, and ghosts are real too. They live inside us, and sometimes, they win.

Writing is socially acceptable form of being naked in public – Paulo Coelho

When I read this quote, I was dumb founded! I always shied away from sharing my writings as I was not comfortable baring my thoughts or feelings..I used to think if someone reads this, what would they think? If I write something about love / pain would they think I have experienced it myself? I was not ready to answer anyone’s questions if they did! I wrote still but not about anything personal. I wrote for my diary/ on Facebook / WhatsApp group /hobby.

But everything was about some snippet seems like political my stuff most of the time. If I posted anything that would look personal political , my inbox would be flooded with oh, what happened, etc.
After I read this quote, I was like if such a great author can feel this way, it was ok for me to feel the same!

It is time now to write about what I really want to write without having any inhibitions..It may not be liked by all…but I would love the freedom of having all the thoughts outside my mind onto my group / fb / article medium.

‘Coz I have also learnt that when you put out all your thoughts on paper it reduces the intensity of your feelings..you become more calm & are at peace with yourself.

I would be very happy to unleash my love / passion for writing & also sharing it with few people who are interested in my work.

So how did it all began?

It all started with my another passion, Reading! Ya right, If I do anything, it has to be passionately! I don’t know any other way to do it 🙂

Reading has always been like a passion for me since childhood. I have always been a voracious reader. It may be newspapers, comics, short stories, novellas or full fledge novels. I forget myself when i am reading.. I become one with the characters & feel their emotions empathetically. It takes me to places where I can not physically go..transporting me to a world of imagination. Reading has also fuelled my already active imagination! The next logical step for me was to then pour out all the imagination in the form of writing..

I used to write poetries, short essays & stories..but I immediately tore them up after writing..(but aftet Apart from the reason above, I was also not sure whether I was a good writer or not..

Getting appreciated for whatever I have written till now has made me more comfortable with sharing my writings with public..

To cut a long story short, it is time to fuel my passion & drive it on the highway of imagination..If I get some friends along with me, cool!! If not, then Ekla Chalo Re!!!

University Practical exams

MY VIVA #1
Hi,
During the last practical exams I shared my experience. I shared certain questions with answers which the examiner asked me during the practicals.even i share what i learn  Even this time, I'm going to share a few questions and answers with you. Here we go,

This time I had  exam (Practicals), number  - microbial genetics and yesterday enzymology in biochemistry and - Molecular biology.
Let us start with the Biochemistry and molecular Biotechnology lab.

1.microbial genetics

What I know about streaking :

Purpose of streaking : 
Why to Streak?

1) To get isolated colonies. ( mostly bacteria )
2) To check the presence of microbes in a sample ( sometimes)

Types:

1) Quadrant streaking: The plate is divided into 4 quadrants and streaking is done.
2) Continuous streaking: Continuous streak made from top to bottom.
After this 2nd experment is of procedure of transformation
I.e. simple
Bacterial Transformation:
1. Pipette 200ul sample cells into each of 3 ice cold Eppendorf tubes. Label the tubes Control, 1 ng, and 10 ng (1 ng is 10 -3 ug, or 10 -9 milligrams). The unknown plasmid is at a concentration of 1 ng/ul. Add 1 ng of your unknown plasmid to one tube and 10 ng to the other. Place the tubes on ice for 30 min.
2. Put the tubes at 42oC for exactly 90 seconds. Return the cells to ice for 1-2 minutes.
3. Pipette the transformation mixtures onto labeled plates containing ampicillin and spread them around using a sterilized, bent glass rod spreader.
4. Place upside down in the 37oC incubator in room 305 overnight.
5. 16 - 20 hours later, count the number of colonies on the plate with well-isolated colonies. Put parafilm around the edge of a plate and put it in a refrigerator for later use. Check the control plate to see that no colonies grew on it.

And the viva voce, that was very simple :) I scored 10/10 :)

Okay, let us see some of the questions that examiner asked me.

1) Question: Which gene responsible for antibiotic resistance
Answer: some plsmid have Ability to make host cell resistance to one or more antibiotic and these also transfer the resistance to cell lack R factor i.e. resistance
Sub questi

2)   Question:  What is plasmid and give some examples
Answer: Plasmid is extracellular DNA in prokaryotic oraganism and some are artificially synthesis plasmid also its genetic material ,
There are several types of plasmid

   F plasmid - fertility plasmid
   R plasmid - resistance plasmid
   col plasmid
   Promiscuous plasmid
   Virulence plasmid

3) Tell me role of STET buffer ??

 sir actually it contains Sucrose which helps in maintaining osmotic pressure; Triton X which helps in cleaving the cell wall; EDTA which acts as a chelating agent; Tris HCl is used as buffer.

nice tell me ur roll no ?
sir it's 69 😊😊

  

Bye meet with new subject .....

Whats Your Favorite Dish

Hi, I am  Cooking for my little one my cute one and love them lot and I love u also

SO what's your favorite dish ?

Everyone has his favorite dish like plants love urea  phosphorus and minerals my garden need manure for my garden

I'm a bit different; I never grow roses,lilies, sunflowers in my garden; But, just cute little microscopic flowers

microbes!!! Each and every person will have their own favorite dish.

Mine is Tandur and butter masala. What's your's?

What do these little cells require for growing well? 
But, what do my microbes need? What's their favorite?
Got an answer!!! Got an answer!

They love Agar!!! Starting with basics of preparing nutrient agar in my lab tomorrow.
I use to ask some questions to my professor and some i search online

Oooooo... My microbes are nonveggy. They love beef extracts, so i must add some to my nutrient agar!

Got some questions :

1) At what temperature does agar liquefy and solidify???

        It's very simple to answer this,
Solidifies at 32 to 40 degree C.
Liquefies at 80 degree C.

2) What role does agar serve as a nutrient ???

         Most microbes can't destroy and feed up agar, it just gives a background for holding the nutrients and the microbes. They give support to the cell wall of certain algae! It's a polymer made of galactose units.

3) Why agar instead of gelatin ?

Gelatin liquefies at even less temperature in the range of 35 to 40 degree C. But, Agar melts at 80 degree Moreover, only few microbes can degrade it , hence it can provide a better solid surface for growing microbes.

4) Nutrient agar for my microbes with contents like :

Glucose ---> Provides carbon                    source.
  Peptone ---> Gives protein
  Beef extract ---> aminoacids, sugars, inorganic salts
  Nacl ---> For maintaining osmotic pressure.
  
  NaCl is generally added to the nutrient media for maintaining the Osmotic pressure. Maintaining osmotic pressure is important, because, increase or decrease in the osmotic pressure leads to cell burst or death due to the effect of osmosis. So, maintaining the osmotic pressure is done by adding correct amount of NaCl. ( I learn this concept in last semester I like to relete science concept in various experiment)

Got tired out of typing and reading my notes and papers. ooov.. Gonna sleep.. good night!!!
will feed you tomorrow my little ones.!!! :P

Suger A sweet villan

Its nice to write here after a long time, how are u all 😊
Nowadays, I'm very much interested in reading something new regarding my subject, "Biotechnology".People say, "oh Tahakik you are bright student, then why you chose Biotechnology?
It has no scope. ,😣😣
You won't earn better. You might have chosen Engineering , Pharmacy or medical , UPSC's.
something like that..." But, who cares 😐💰💰
I'm loving my subject. Biotechnology is the branch where you can survive only when you are much interested. anyway
I'm thirsty , reading a lot for my ongoing and new research project. But, I'm finding it sometimes much difficult to organize and start with. I'm getting hundreds of ideas, but, I find some of them silly, I find  some of them  complicated, I find  some of them  impossible! But, still, with hope in myself and interest in the subject, I'm continuing with them spend lots of time to think and playing with ideas ...
My dream is to  find something new and publish an article in a high impact journal.
Okay, let's stop here with my story, and go with the main matter. (sry for this )
We all love sweets, isn’t it? From a cute baby who had just started feeling and enjoying the tastes to 100 year old people, love sweets! 😘😋

Oh, I could hear you, yes, "Diabetic patients"
can’t enjoy sweets.
But, they too love to have sweets. Even, my Grandfather is diabetic and he uses to eat sweets hiding from my gramdmaa
Okay, what for that sweet love,🌷🌷
you are asking? Nothing wrong in that, but, if you are crazy about the bottled drinks, then, I’m sorry, you must start worrying!

Aspartame – is an artificial sweetener which is used in most of the bottled drinks. And, that is an approved artificial sweet nerby FDA (US Food and Drug Administration –which tests and approves all food and drug items before they are marketed)

“It is approved by FDA, then, why we must worry?”, is this your next question? Let me answer you!Aspartame is formed using two

aminoacids –
L-Phenyl alanine
L aspartate.

This when broken down during metabolism in ourbody produces – phenylalanine and hence must be avoided by people who are suffering from the genetic disorder called Phenyl Ketone Urea (PKU) – these people can’t metabolise phenyl alanine further!

This Aspartame will be unstable at higher temperatures and they are stable in the pH of 4 in general. As most of the bottled drinkshave the pH of 3 to 5, this is generally used in bottled drinks.(prefer chilled)

Moreover, this is preferred because this has 200% greater sweetness than the normal sucrose we use.

This is enough for us right?
The taste? The taste is enough, right?Okay, what is the problem in consuming thisaspartame? Only the PKU people must avoid this?

No, even normal healthy individual’s health will be affected on consuming aspartame in long run – many researches revealed this!

There are several published articles narrating the link between the aspartame consumption and Cancer! Yes, cancer,

leukemia!In 1965 Aspartame was discovered and was approved by FDA initially in 1974. Even after the approval, the safety of aspartame is being questioned by many researchers and the FDA considered the approval again!A trick to hide the risks behind a product is generally done by showing the results of short term tests and analysis. People are misled by such short term analysis!The metabolized products of aspartame not only include aspartate and phenyl alanine but also methanol. Methanol is toxic! But, many scientists and researches argue that methanol could be degraded by alcohol dehydrogenases. But, this degradation results in formaldehyde which will degrade DNA and proteins! This will lead to several Auto immune diseases and cancer!

And, researches reveal that men are affected more by the consumption of aspartame!
Many products like this, which are synthesized artificially might have long termeffects over our health, as this is going to affect us only in long run we won’t realize this.My mom use to advice me not to take bottled drinks and she suggests fresh juices instead (By Patanjali 😂😂).

She says, if I drink bottled items, it would affect my health.
It’s true isn’t it? Aspartame or other artificial sweeteners might be the reason behind this?I’m not sure about the worst effects of Aspartame, I just wrote this post by reading various articles and information already published.You are sick? May be it is due to artificial sweeteners? To check this, just avoid artificial sweeteners. Check the label of everything you consume sweet! If you are fine after avoiding these artificial sweets, then, the problem is with the artificial sweeteners.Just try to be safe with natural sweets!

ग्रुप्स आणि मी

प्रत्येकाला काही न काही अडचणी असतात. कोणी बोलतं, कोणी बोलत नाही. कोणी आपापले मार्ग आपणच शोधतात, कोणी इतरांना विचारतात.

पण ह्या सगळ्या प्रोसेस मधे सोबत कोणीतरी हवं असतं. नेहमी सगळा प्रॉब्लेम शेअर केला जाईल असही नाही. पण नुसतं बोलायला, व्हेंट आऊट व्हायला हवं असतं.

कधी माहिती - मदतहवी असते. या सगळ्यामधे प्रत्येकाच्या कॉंट्रीब्युशन असते, असावी लागते. कधी नुसतं एेकून घ्यायची, कधी सल्ले देण्याची,
*टर्न बाय टर्न हे प्रत्येकानं केलं तर गृप घट्ट होत जातो*

कधी एखादीला सतत माहितीच हवी, ती मात्र इतरांच्या मदतीला येत नाही, असं झालं की मग हा बंध सैल होऊ लागतो. सतत एकीलाच पँपर करत रहाणं हेही काही फार संयुक्तिक नाही.

तसच कधीतरी तुमची चूक दाखवली जाऊ शकते हेही लक्षात घ्यायला हवं.

तर कधी कोणीच मदतीला येऊ शकत नाही हेही समजून घेतलं पाहिजे.

प्रत्येकाचं मत वेगळं, अनुभव वेगळा, स्वभाव वेगळा,... हे प्रत्येकानेच समजून घ्यायला हवं.
आणि समजा एखाद्याला नाही समजलं एखाद वेळेस तर ते सोडूनही देता आलं पाहिजे.

सर्वात महत्वाचे म्हणजे गृप मधून बाहेर पडताना, नीट सांगून बाहेर पडायला हवं. परतीची वाट ओपन ठेवा.

हे सगळं लिहितोय ते खर तर माझं मलाच सांगतोय. सो नो ऑफेन्स प्लिज

इत्यादी...

तुला मिठीत घ्यायला
मला ' आवडणार ' नाही...
आवडत नाही असे कुणी सांगितले ?
तसे फार वेळा मनात येते
पण मग तिथे गोष्टी संपत नाहीत
मिठी संभाषणासारखी असते
आधी साधत नाही
मग कधी संपत नाही
आता त्या न संपणाऱ्या न घडणाऱ्या
संवादाचे काय करावे?
मिठी रेंगाळनार्या संवादात
'इत्यादी' असतोच ना
तसे तू मिठीचे करून टाकतेस
इत्यादी इत्यादी घुसमटनारे इत्यादी
दिघ:मूढ इत्यादी शब्द,स्पर्श,व्याकुळ ,
इत्यादी इत्यादींच्या अनेक तऱ्हां......
इत्यादींची मला भीती वाटते
तुलाही वाटते
तुझीही वाटते
इत्यादी भीती वाटण्यासाठीच असतात
किंवा निरर्थकमध्येच
केंव्हा तरी
माझ्याकडे वळणार्या
तुझ्या आतुर नजरेसारखी
ती निरर्थक असते !!!
असे नव्हे ,
पण कदाचीत तसेच असते
कारण ती इत्यादी असते
इत्यादी म्हणले तर अर्थपूर्ण असतात
म्हणले तर निरर्थक
मग कुणी म्हणले तर......
इत्यादी,इत्यादी........

(तहकीक ऋषिकेश रवींद्र)

BROKEN WORDS

कविता इथेच संपल्याचं 
मधेच कऴल्यावर 
पुढल्या सगळ्या ओळी 
खोडतांना
आठवत राहातात
अनेक संबंध 
ज्यांच्यातलं कवित्व संपलं होतं
कधीच पण
लिहित राहिलो होतो 
त्यांच्याअनावश्यक ओळी आपणच
अपरिहार्यपणे
कागदावरल्या ओळी 
कापता तरी आल्या 
पण संबंधांना तसंच ठेवलं तरंगत 
आपण निर्दयपणे 
वेगवेगळ्या कवितांच्या
गल्ल्यांतून चकवा लागल्यात
पुन्हा पुन्हा पोहोचलो 
त्याच हमरस्त्यांवर तरी संबंधांवरली शीर्षकं कायमची खोडता नाही आली
आपल्यालाआणि चांगल्या ओळी 
वेगळ्या काढून 
संबंधही Re write करता आले नाही 
अज़ून नशेत लिहिलेल्या
कित्येक ओळिंचे संदर्भदुसर्या दिवशी लागता लागले नाहीत 
किंवा खपली खालच्या साकळल्या 
ओळीही भळभळल्या नाहित 
कधीच तरी संबंधांचे 
व्रण मात्र कायमचे उठतच राहिले आपल्यावरआता
कधीतरी 
आज़वर कापलेल्या ओळी 
आणि 'उगाच' वाढलेल्या संबंधांना 
"मांज़रीचं" गोंडस् पिल्लू 
समज़ून अंधार्या पठारावर यायला हवं सोडून

आपापल्या पायांवर 
त्यांनी स्वताःच 
शेवटी मोठं व्हायला हवं म्हणून....!!